Nomenclature of Legacy pCAMBIA vectors

The four digit numbering system works as follows:

• First digit – indicates plant selection: 0 for absence; 1 for hygromycin resistance; 2 for kanamycin; and 3 for phosphinothricin (the vectors containing the phosphinothricin resistance gene are no longer available from CAMBIA at the request of Bayer, which owns patents restricting its use in some countries).
• Second digit – indicates bacterial selection: 1 for spectinomycin/streptomycin resistance; 2 for chloramphenicol; 3 for kanamycin; 4 for spec/strep and kanamycin.
• Third digit – indicates polylinker used: 0 for pUC18 polylinker; 8 for pUC8 polylinker; 9 for pUC9 polylinker.
• Fourth digit – indicates reporter gene(s) present: 0 for no reporter gene; 1 for E.coli gusA; 2 for mgfp5; 3 for gusA:mgfp5 fusion; 4 for mgfp5:gusA fusion; 5 for Staphylococcussp. gusA (GUSPlus).
• Fifth digit – notes some other special feature. So far this has been used only with: pCAMBIA1305.1 and plasmids derived from it, where the .1 denotes the absence of a signal peptide from the GUSPlus™ protein; and pCAMBIA1305.2 where the .2 denotes the presence of the GRP signal peptide for in planta secretion of the GUSPlus™ protein.
• Lagging letter – X indicates that the reporter gene lacks its own start codon and the vector is for creating fusions to the reporter; Z indicates presence of a functional lacZa for blue-white screening; a/b/c indicates the reading frame for fusions with the Fuse and Use vectors.

Notes

• Due to resource limitations, not all possible vector feature combinations have been created at CAMBIA. You may initially be disappointed to find that we don’t have, for example, a pCAMBIA2205.2. The vectors were designed however, such that it should be a relatively simple matter for a researcher needing such a vector to construct it from the components in other vectors.
• If you have created a pCAMBIA vector derivative that other researchers will find useful, please let us know .
• Vector Manual Addendum: Important note to pCAMBIA users

Quick-pick table

(clicking on a plasmid number will navigate to the Genbank file)

pCAMBIA	Plant Selection Gene	Bacterial Selection Gene	Polylinker	Reporter Gene	T-DNA size (bp)	Reading Frame or lacZ	Vector family	Map
2301	nptII	kan	pUC18	gusA	5391	–	GIS	view map
2300	nptII	kan	pUC18	–	2512	–	MSV	view map
2201	nptII	cmr	pUC18	gusA	5391	–	GIS	view map
2200	nptII	cmr	pUC18	–	2512	–	MSV	view map
1405.1	hptII	kan+ spec/strep	pUC18	gus plus	5593	lacZ	GIS	view map
1391	hptII	kan	pUC9	gusA	4427	–	FUV	view map
1391Z	hptII	kan	pUC9	gusA	4984	lacZ	PCV	view map
1391Xc	hptII	kan	pUC9	gusA	–	c	FUV	view map
1391Xb	hptII	kan	pUC9	gusA	–	b	FUV	view map
1391Xa	hptII	kan	pUC9	gusA	–	a	FUV	view map
1390	hptII	kan	pUC9	–	2630	–	MSV	view map
1381	hptII	kan	pUC8	gusA	4427	–	FUV	view map
1381Z	hptII	kan	pUC8	gusA	4984	lacZ	PCV	view map
1381Xc	hptII	kan	pUC8	gusA	–	c	FUV	view map
1381Xb	hptII	kan	pUC8	gusA	–	b	FUV
1381Xa	hptII	kan	pUC8	gusA	–	a	FUV	view map
1380	hptII	kan	pUC8	–	2630	–	MSV	view map
1304	hptII	kan	pUC18	gfp:gusA	6128	–	GFP	view map
1303	hptII	kan	pUC18	gusA:gfp	6128	–	GFP	view map
1302	hptII	kan	pUC18	gfp	4316	–	GFP	view map
1301	hptII	kan	pUC18	gusA	5607	–	GIS	view map
1300	hptII	kan	pUC18	–	2728	–	MSV	view map
1291Z	hptII	cmr	pUC9	gusA	4984	lacZ	PCV	view map
1281Z	hptII	cmr	pUC8	gusA	4984	lacZ	PCV	view map
1201	hptII	cmr	pUC18	gusA	5606	–	GIS	view map
1200	hptII	cmr	pUC18	–	2727	–	MSV	view map
0390	–	kan	pUC9	–	582	–	DIY	view map
0380	–	kan	pUC8	–	582	–	DIY	view map