In transactivation, upstream activation sequences (UAS) are inserted into plants separately from the transcriptional activators which regulate them. This enables the transcriptional activators to create changes in gene regulation by either inducing or suppressing the expression of certain genes even though they are not linked to the promoters of these genes.
Many investigators have worked with transactivation (see the February 2006 issue of Plant Journal for a recent review), but at CAMBIA our focus shifted to developing a system free of the IP constraints of the systems widely used in Arabidopsis and Drosophila, to enable people to get around barriers to producing technology that is actually deliverable (see Connett and Jefferson 2005).
Use of transactivator sequences presents a method for coordinate expression of multiple genes in a pathway. It may also present a means to “work around” patents that block use of many promoters “operably linked” to genes of interest.
Transactivation research at CAMBIA has been done principally in rice (funded by the Rural Industries RDC and in the Rockefeller Foundation funded work of several Ph.D. students, some still being examined by the relevant thesis committees, but available by agreement). Potential applicability extends to many species and we encourage you to contact us if you are interested in using and improving these sequences in your species of interest.
What is available in this project?
- How transactivation works
- Using Transactivation Lines
- Potential uses of transactivators
- IP Issues and Transactivation
- Vector NTI diagrams
- Full Technical Reports
Ordering Transactivation Vectors
Unfortunately, CAMBIA is no longer able to distribute materials. Please contact us to find out if there are any labs which may be able to share materials with you.
Notes on plant materials:
CAMBIA is currently completing an inventory for databasing its transactivator lines in rice, many with molecular analysis showing segregation patterns up to three generations. Collaborative projects have already been initiated with research groups in China, Indonesia and Vietnam. We can make seed available from UAS lines in which the gene expression pattern may be of interest to regulate by crossing with a transactivator line. If you are interested even before the inventory is complete or have ideas toward improvements in the database, please contact CAMBIA.