Donor Vectors

Background of Donor Vectors

The term “donor vector” may be used to refer to a vector that contains Gateway modifications. These modifications include the presence of attP recombination sequences. When used in conjuction with a specific recombinase (these vectors allow integration of other DNA or PCR products containing attB recombination sequences. One of the products of such a reaction is a new vector containing the integrated DNA of interest, flanked by attL recombination sequences. This new vector is often referred to as an “entry clone”. The attL-flanked sequences within entry clones may then be moved via an LR recombination reaction into further gateway vectors containing attR sequences.

Hence donor vectors are particularly useful in cloning of PCR products and are often used to produce entry vectors for further manipulation. Commercial (Invitrogen) donor vectors are often give the prefix pDONR. Some common examples are: