Positive selection using glucosamine-6-phosphate
Danisco A/S holds granted patents in the United States, Australia and New Zealand regarding the method for the positive selection of genetically transformed plant cells by glucosamine 6-phosphate.
To normal plant cells, glucosamine or its derivatives such as glucosamine 6-phosphate, is a nutrient when present in a low concentration, However, they become toxic when their concentration exceeds certain level.
Glucosamine 6-phosphate deaminase from Escherichia coli is an allosteric hexameric enzyme which catalyzes the reversible conversion of D-glucosamine-6-phosphate into D-fructose 6-phosphate and ammonium ion and is activated by N-acetyl-D-glucosamine 6-phosphate. By transforming a gene coding for the glucosamine 6-phosphate deaminase into plant cells, the transformed cells will acquire the capability of converting glucosamine-6-phosphate to fructose-6-phosphate and ammonium. In this way, the transformed plant cells can use glucosamine-6-phosphate at a high concentration as a source of both carbohydrate and nitrogen for their growth over the non-transformed cells.
Detailed patent information
|Patent number||Title, Independent Claims and Summary||
||Title – Method of plant selection using glucosamine-6-phosphate deaminase
|AU 739067 B2
||Title – Selection method for transgenic plant
|Related patent was also granted in New Zealand (NZ 336925). The European application (EP 970221 A1) was deemed to be withdrawn on 30 Dec 2005. Patent applications were also filed in Canada (CA 2280236), China (CN 1252097) and Japan (JP 2002514915).|
|Date of search||30/05/2006|
|Database searched||Patent Lens|
|Type of search||Simple, stemming on|
|Collections searched||AU-B, US-A, US-B, EP-B, WO|
|Search terms||“Danisco” in applicant
“cell selection” in abstract
|Comments||Both US 6444878 and AU 739067 B2 were listed|