Nomenclature of pCAMBIA GusPlus vectors

The numbering system works as follows:

• First digit – indicates plant selection: 0 for absence; 1 for hygromycin resistance; 2 for kanamycin.
• Second digit – indicates bacterial selection: 1 for spectinomycin/streptomycin resistance; 2 for chloramphenicol; 3 for kanamycin; 4 for spec/strep and kanamycin.
• Third digit – indicates polylinker used: 0 for pUC18 polylinker; 8 for pUC8 polylinker; 9 for pUC9 polylinker.
• Fourth digit – indicates reporter gene(s) present: 0 for no reporter gene; 1 for E.coli gusA; 2 for mgfp5; 3 for gusA:mgfp5 fusion; 4 for mgfp5:gusA fusion; 5 for Staphylococcus sp. gusA (GUSPlus).
• Fifth digit – the .1 denotes the absence of a signal peptide from the GUSPlus protein and the .2 denotes the presence of the GRP signal peptide for in planta secretion of the GUSPlus protein.

Quick-pick table (clicking on a plasmid number will navigate to the Genbank file)