Nomenclature of pCAMBIA GusPlus vectors
The numbering system works as follows:
- First digit – indicates plant selection: 0 for absence; 1 for hygromycin resistance; 2 for kanamycin.
- Second digit – indicates bacterial selection: 1 for spectinomycin/streptomycin resistance; 2 for chloramphenicol; 3 for kanamycin; 4 for spec/strep and kanamycin.
- Third digit – indicates polylinker used: 0 for pUC18 polylinker; 8 for pUC8 polylinker; 9 for pUC9 polylinker.
- Fourth digit – indicates reporter gene(s) present: 0 for no reporter gene; 1 for E.coli gusA; 2 for mgfp5; 3 for gusA:mgfp5 fusion; 4 for mgfp5:gusA fusion; 5 for Staphylococcus sp. gusA (GUSPlus).
- Fifth digit – the .1 denotes the absence of a signal peptide from the GUSPlus protein and the .2 denotes the presence of the GRP signal peptide for in planta secretion of the GUSPlus protein.
Quick-pick table (clicking on a plasmid number will navigate to the Genbank file)
pCAMBIA | Plant Selection Gene | Bacterial Selection Gene | Polylinker | Reporter Gene | T-DNA size (bp) | Reading Frame or lacZ | Vector family | Map |
---|---|---|---|---|---|---|---|---|
1305.2 | hptII | kan | pUC18 | gus plus with secretion signal peptide | 5667 | – | GIS | view map |
0305.2 (identical to above vector but contains no hptII cassette) |
– | kan | pUC18 | gus plus with secretion signal peptide | 3412 | lacZ | GIS | |
1305.1 | hptII | kan | pUC18 | gus plus | 5592 | – | GIS | view map |
0305.1 (identical to above vector but contains no hptII cassette) |
– | kan | pUC18 | gus plus | 3408 | lacZ | GIS | view map |
1105.1 | hptII | spec/strep | pUC18 | gus plus | 5854 | lacZ | GIS | view map |
1105.1RC | hptII | spec/strep | MCS with rare cutting enzymes | gus plus | 3654 | GIS | (view map) |
|
1105.1U | hptII | spec/strep | unique MCS | gus plus | 5830 | view map | ||
1105.1R (download as attachment) |
hptII | spec/strep | modified MCS | gus plus | 5915 | – | GIS | view map |
1105.1RRC | hptII | spec/strep | MCS with rare cutting enzymes | gus plus | 3665 | GIS | view map |
|
0105.1R (identical to above vector but contains no AseI-hptII cassette, but if you have VectorNTi program you can download the attached sequence as gb.file) |
– | spec/strep | gus plus | 3489 | – | GIS | view map |