Nomenclature of pCAMBIA GusPlus vectors

The numbering system works as follows:

  • First digit – indicates plant selection: 0 for absence; 1 for hygromycin resistance; 2 for kanamycin.
  • Second digit – indicates bacterial selection: 1 for spectinomycin/streptomycin resistance; 2 for chloramphenicol; 3 for kanamycin; 4 for spec/strep and kanamycin.
  • Third digit – indicates polylinker used: 0 for pUC18 polylinker; 8 for pUC8 polylinker; 9 for pUC9 polylinker.
  • Fourth digit – indicates reporter gene(s) present: 0 for no reporter gene; 1 for E.coli gusA; 2 for mgfp5; 3 for gusA:mgfp5 fusion; 4 for mgfp5:gusA fusion; 5 for Staphylococcus sp. gusA (GUSPlus).
  • Fifth digit – the .1 denotes the absence of a signal peptide from the GUSPlus protein and the .2 denotes the presence of the GRP signal peptide for in planta secretion of the GUSPlus protein.

Quick-pick table (clicking on a plasmid number will navigate to the Genbank file)

pCAMBIA Plant Selection Gene Bacterial Selection Gene Polylinker Reporter Gene T-DNA size (bp) Reading Frame or lacZ Vector family Map
1305.2 hptII kan pUC18 gus plus with secretion signal peptide 5667 GIS view map
0305.2
(identical to above vector but contains no hptII cassette)
kan pUC18 gus plus with secretion signal peptide 3412 lacZ GIS
1305.1 hptII kan pUC18 gus plus 5592 GIS view map
0305.1
(identical to above vector but contains no hptII cassette)
kan pUC18 gus plus 3408 lacZ GIS view map
1105.1 hptII spec/strep pUC18 gus plus 5854 lacZ GIS view map
1105.1RC hptII spec/strep MCS with rare cutting enzymes gus plus 3654 GIS
(view map)
1105.1U hptII spec/strep unique MCS gus plus 5830 view map
1105.1R
(download as attachment)
hptII spec/strep modified MCS gus plus 5915 GIS view map
1105.1RRC hptII spec/strep MCS with rare cutting enzymes gus plus 3665 GIS
view map
0105.1R
(identical to above vector but contains no AseI-hptII cassette, but if you have VectorNTi program you can download the attached sequence as gb.file)
spec/strep gus plus 3489 GIS view map