Appendices 3. Bar genes from Streptomyces viridochromogenes

The following patents were originally assigned to Hoechst AG and are now part of the Bayer Crop Science portfolio.

Actual granted independent claims.

EP 257542 B1
Claims for the following contracting states: BE, CH, DE, FR, GB, GR, IT, LI, LU, NL, SE
Claim 1
A phosphinothricin (PTC) resistance gene which can be obtained by cutting, with BamHI, the total DNA from Streptomyces viridochromogenes DSM 4112 which has been selected for phosphinothricyl-alanyl-alanine (M) resistance, by cloning fragment 4.0 kb in size, and by selection for PTT resistance.
Claims for the following contracting states: AT, ES
Claim 1 
A process for obtaining a phosphinothricin (PTC) resistance gene which comprises isolation of a fragment 4.0 kb in size by cutting, with BamHI, from the total DNA from Streptomyces viridochromogenes DSM 4112 which has been selected for phosphinothricyl-alanyl-alanine (M) resistance, cloning the whole of this fragment or a part thereof containing the resistance gene, and selecting for PTT resistance.
EP 275957 B1
Claims for the following contracting States: AT, BE, CH, DE, FR, GB, GR, IT, LI, LU, NL, SE
Claim 1
A resistance gene coding for the protein of amino acid sequence 1 (annex), in that ATG is used as start codon and TGA is used as stop codon, and the GC content of the gene is adapted to that in plants.
Claim 7
A host cell containing a vector as claimed in claim 4, 5 or 6.
Claim 8
A plant cell containing a gene as claimed in claim 1, 2 or 3.
Claims for the following contracting state: ES
Claim 1
A process for the preparation of a phosphinothricin (PTC)-resistance gene, which comprises synthesis of a gene which codes for the protein of amino acid sequence 1 (annex), wherein ATG is used as start codon and TGA is used as stop codon, and the GC content of the gene is adapted to that in plants.
Claim 3
A process for the generation of PTC-resistant plant cells, plants, parts of plants or seeds, which comprises coupling a gene which has been obtained as claimed in claim 1 or 2 to regulation and expression signals active in plants, introducing the resulting gene structure into plant cells, and bringing about its expression therein.
US 5273894
Claim 1
Phosphinothricin (PTC)-resistance gene obtainable by selecting Streptomyces viridochromogenes DSM 4112 for resistance to phosphinothricyl-alanyl-alanine (PTT), cutting with BamHI the total DNA from the resistant strains, cloning a fragment 4.0 kb in size, and selecting for PTT resistance.
Claim 4
A plant cell transformed with the gene of claim 2.
Claim 5The gene as claimed in claim 1, comprising at least the positions 258806 of the DNA sequence I: [sequence omitted]
Claim 8
A bacterium transformed with the gene of claim 1.
Claim 9
A plant cell transformed with the gene of claim 1.
Claim 10
A process for the selective acetylation of the NH2-group of the L-form of racemic PTC which comprises contacting racemic PTC with
(a) a cell expressing a phosphinothricin (PTC)-resistance gene, wherein said gene is obtainable by selecting Streptomyces viridochromogenes DSM 4112 for resistance to phosphinothricyl-alanyl-alanine (PTT), cutting with BamHI the total DNA from the resistant strains, cloning a fragment 4.0 kb in size, and selecting for PTT resistance, or
(b) the enzyme encoded by said gene and fractionating the D-form and the acylated L-form of PTC.
US 5276268
Claim 1
An isolated resistance gene coding for the protein of amino acid sequence III, which gene is adapted to codon usage in plants so that it is expressed in plant cells at a level sufficient to confer resistance to phosphinothricin in said plant cells.
Claim 3
A gene structure having DNA sequence III operatively linked to regulation and expression signals active in plants so that it is expressed in plant cells at a level sufficient to confer resistance to phosphinothricin in said plant cells.
Claim 6
A plant cell containing a gene as claimed in claim 1.
Claim 9
Plants and their propagules containing a gene as claimed in claim 1.
Claim 12
A process for generating phosphinothricin resistant plant cells, plants, and their propagules which comprises transforming plant cells with the gene as claimed in claim 1, and regenerating the transformed plant cells to plants which produce propagules.
US 5637489
Claim 1
A process for the production of a PTC-resistant plant which comprises incorporating into the genome of the plant a phosphinothricin (PTC)resistance gene obtainable by selecting Streptomyces viridochromogenes DSM 4112 for resistance to phosphinothricylalanyl-alanine (PTT), cutting with BamHI the total DNA from the resistant strains, cloning a fragment 4.0 Kb in size, and selecting for PTT resistance.
Claim 3
A process for the production of a PTC-resistant plant which comprises incorporating into the genome of the plant a phosphinothricin (PTC)resistance gene obtainable by selecting Streptomyces viridochromogenes DSM 4112 for resistance to phosphinothricylalanyl-alanine (PTT), cutting with BamHI the total DNA from the resistant strains, cloning a fragment 4.0 Kb in size, and selecting for PTT resistance, wherein the PTC-resistance gene so-obtained contains at least positions 258-806 of the DNA sequence I [sequence omitted here].
Claim 4
A plant which contains the PTC-resistance gene said gene is obtainable by selecting Streptomyces viridochromogenes DSM 4112 for resistance to phosphinothricyl-alanyl-alanine (PTT), cutting with BamHI the total DNA from the resistant strains, cloning a fragment 4.0 Kb in size, and selecting for PTC resistance.
US 5879903
Claim 1
A method of using a phosphinothricin (PTC)-resistance gene as a resistance marker in bacteria comprising the steps of transforming the bacteria with the phosphinothricin (PTC)-resistance gene, culturing the bacteria in a medium, exposing the bacteria to phosphinothricin, and determining whether the bacteria is resistant to the phosphinothricin, wherein the phosphinothricin (PTC)-resistance gene is obtainable by selecting Streptomyces viridochromogenes DSM 4112 for resistance to phosphinothricyl-alanyl-alanine (PTT), cutting with BamHI the total DNA from the resistant strains, cloning a fragment 4.0 kb in size, and selecting for PTT resistance.
Claim 4
A method of using a phosphinothricin (PTC)-resistance gene as a resistance marker in plant cells comprising the steps of transforming the plant cells with the phosphinothricin (PTC)-resistance gene, culturing the plant cells in a medium, exposing the plant cells to phosphinothricin, and determining whether the plant cells are resistant to the phosphinothricin, wherein the phosphinothricin (PTC)resistance gene is obtainable by selecting Streptomyces viridochromogenes DSM 4112 for resistance to phosphinothricylalanyl-alanine (PTT), cutting with BamHI the total DNA from the resistant strains, cloning a fragment 4.0 kb in size, and selecting for PTT resistance.