Appendices 4. Bar genes from Alcaligenes sp and other gram-negative bacteria

The third patent family making up the Bayer Crop Science portfolio. These patents were originally assigned to Hoechst AG.

Granted actual claims.

EP 290986 B1
Claims for the following contracting states : CH, DE, FR, GB, IT, LI, NL
Claim 1
A phosphinothricin(PTC)-resistance gene, obtainable by selection of bacteria, which are not fungus-like, for PTC resistance, extraction of the DNA, construction of a gene bank, isolation of PTC-resistant clones, and extraction of the PTC-resistance gene from these clones.
Claim 2
A gene as claimed in claim 1, wherein bacteria of the genus Pseudomonas, Alcaligenes, Agrobacterium, Enterobacter, Serratia or Cedecea bacteria of the species Pseudomonas paucimobilisAlcaligenes faecalis or eutrophus, Agrobacterium tumefaciens, Enterobacter agglomerans, Serratia plymuthica or Cedecea Gr. V are used.
Claim 8
Useful plants and parts thereof, which harbor an expressible resistance gene as claimed in one or more of claims 1 to 5.
Claim 9
Propagation material from useful plants, which harbors an expressible resistance gene as claimed in one or more of claims 1 to 5.
Claim 10
The use of microorganism populations which express the resistance gene as claimed in one or more of claims 1 to 5 in sewage treatment plants or on areas under agricultural use
Claims for the following contracting state : ES
Claim 1
A process for obtaining a phosphinothricin(PTC)-resistance gene, which comprises selecting bacteria which are not fungus-like for PTC resistance, isolating the DNA from the selectants, constructing a gene bank therefrom, isolating PTC-resistant clones, and obtaining the resistance gene from the DNA thereof.
Claim 2
The process as claimed in claim 1, wherein bacteria of the genus Pseudomonas, Alcaligenes, Agrobacterium, Enterobacter, Serratia or Cedecea are used.
Claim 6
A process for the production of PTC-resistant plant cells, plants, parts of plant and propagation material, which comprises inserting into the plant cells an expressible gene which has been obtained as claimed in claim 1 to 5.
Claim 7
A process for the selection of plants, plant cells or bacteria, which comprises inserting into the cells an expressible gene which has been obtained as claimed in claim 1 to 5, and selection for PTC resistance.
Claim 8
A process for the biological degradation of PTC in PTC-containing residues, areas under agricultural use, and sewage, which comprises contacting the residues or sewage with microorganism populations which express a gene obtained as claimed in claim 1 to 5.
US 5077399
Claim 1
A phosphinothricin (PTC)-resistance gene obtained by selecting bacteria from the genus Alcaligenes for PTC resistance, extracting the DNA, constructing a gene bank, isolating PTC-resistant clones, and obtaining the PTC-resistance gene from these clones.
Claim 2
A gene as claimed in claim 1, wherein bacteria of the species Alcaligenes faecalis or eutropus are selected.