US 5646024

The following patent (US 5646024) complements the dominant bar gene patents by claiming a process involved in producing transgenic plants resistant to a glutamine synthetase inhibitor.

US 5646024
Title Genetically engineered plant cells and plants exhibiting resistance to glutamine synthetase inhibitors, DNA fragments and recombinants for use in the production of said cells and plants
Issue Date 8 Jul 1997 (17 year term)
Assignees Bayer Crop Science
Inventors Leemans J, Botterman J, de Block M, Thompson C, Mouva R
Appl No and Filing Date US 08/463,241 5 June 1995
Priority US 07/131,140 05-11-1987 (WO 87/05629); divisional application of US 07/525,300 17-051990;US 08/463,241 05-06-1995; EP 86/400521 1103-1986; EP 87/400141 21-01-1987
Abstract The invention relates to a DNA fragment containing a determined gene, the expression of which inhibits the antibiotic and herbicidal effects of Bialaphos and related products. It also relates to recombinant vectors, containing such DNA fragment, which enable this protective gene to be introduced and expressed into cells and plant cells.

As recited in Claim 1, resistance is achieved by introducing a gene obtained from a microorganism that produces the glutamine synthetase inhibitor encoding an acetyl transferase capable of inactivating phosphinothricin or another compo und. In additional claims the introduced gene is the bar gene isolated from either Streptomyces hygroscopicus or Streptomyces viridochromogenes (Claims 2-4).

Other claims recite processes of weed and fungal control, and plants transformed with the bar gene that are cultivated in a field which is subsequently treated with herbicide containing glutamine synthetase inhibitor.

Claim 38 recites a process for the production of a pure culture of transformed plant cells using the glutamine synthetase inhibitor as a means of selection.