Claims of Patents granted to Monsanto

Antibiotic resistance genes in general Patents granted to Monsanto

Actual granted independent claims

US 5034322
Claim 1A chimeric gene capable of expressing a polypeptide in plant comprising in sequence: a) a promoter region from a gene selected from the group consisting of an Agrobacterium tumefaciens opine synthase gene and a ribulose-1,5-bis-phosphate carboxylase small subunit gene; b) a structural DNA sequence encoding a polypeptide that permits the selection of transformed plant cells containing said chimeric gene by rendering said plant cells resistant to an amount of an antibiotic that would be toxic to non-transformed plant cells, said structural DNA sequence being heterologous with respect to the promoter region; and c) a 3′ non-translated region of a gene naturally expressed in plants, said region encoding a signal sequence for polyadenylation of mRNA.
Claim 6 A chimeric gene comprising in sequence: a) a promoter region from a gene selected from the group consisting of an Agrobacterium tumefaciens opine synthase gene and a ribulose-1,5-bis-phosphate carboxylase small subunit gene; b) a heterologous structural DNA sequence encoding a neomycin phosphotransferase; and c) a 3′ non-translated region of a gene naturally expressed in plant cells, said region encoding a signal sequence for polyadenylation of mRNA.
Claim 31 A microorganism identified by ATCC Accession Number 39264.

US 5034322

Claim 1A chimeric gene capable of expressing a polypeptide in plant comprising in sequence:

a) a promoter region from a gene selected from the group consisting of an Agrobacterium tumefaciens opine synthase gene and a ribulose-1,5-bis-phosphate carboxylase small subunit gene;
b) a structural DNA sequence encoding a polypeptide that permits the selection of transformed plant cells containing said chimeric gene by rendering said plant cells resistant to an amount of an antibiotic that would be toxic to non-transformed plant cells, said structural DNA sequence being heterologous with respect to the promoter region; and
c) a 3′ non-translated region of a gene naturally expressed in plants, said region encoding a signal sequence for polyadenylation of mRNA.

Claim 6
A chimeric gene comprising in sequence: a) a promoter region from a gene selected from the group consisting of an Agrobacterium tumefaciens opine synthase gene and a ribulose-1,5-bis-phosphate carboxylase small subunit gene;
b) a heterologous structural DNA sequence encoding a neomycin phosphotransferase; and
c) a 3′ non-translated region of a gene naturally expressed in plant cells, said region encoding a signal sequence for polyadenylation of mRNA.

Claim 31
A microorganism identified by ATCC Accession Number 39264.

US 6174724
Claim 1A chimeric plant-expressible gene, said gene comprising in the 5′ to 3′ direction: a) a promoter region derived from a gene that is naturally expressed in a plant cell and that is capable of effecting mRNA transcription in the selected plant cell to be transformed, operably linked to b) a structural DNA sequence encoding a polypeptide that permits the selection of transformed plant cells containing said chimeric gene by rendering said transformed plant cells resistant to an amount of an antibiotic that would be toxic to non-transformed plant cells, operably linked to c) a non-translated region of a gene naturally expressed in plant cells, said region encoding a signal sequence for polyadenylation of mRNA.
Claim 8A chimeric gene capable of expressing a polypeptide in plant cells comprising in sequence: a) a promoter region from a gene which is naturally expressed in plant cells; b) a 5′ non-translated region; c) a structural coding sequence encoding a neomycin phosphotransferase polypeptide; and d) a 3′ non-translated region of a gene naturally expressed in plant cells, said region encoding a signal sequence for polyadenylation of mRNA.

US 6174724

Claim 1A chimeric plant-expressible gene, said gene comprising in the 5′ to 3′ direction:
a) a promoter region derived from a gene that is naturally expressed in a plant cell and that is capable of effecting mRNA transcription in the selected plant cell to be transformed, operably linked to
b) a structural DNA sequence encoding a polypeptide that permits the selection of transformed plant cells containing said chimeric gene by rendering said transformed plant cells resistant to an amount of an antibiotic that would be toxic to non-transformed plant cells, operably linked to
c) a non-translated region of a gene naturally expressed in plant cells, said region encoding a signal sequence for polyadenylation of mRNA.

Claim 8A chimeric gene capable of expressing a polypeptide in plant cells comprising in sequence:
a) a promoter region from a gene which is naturally expressed in plant cells;
b) a 5′ non-translated region;
c) a structural coding sequence encoding a neomycin phosphotransferase polypeptide; and
d) a 3′ non-translated region of a gene naturally expressed in plant cells, said region encoding a signal sequence for polyadenylation of mRNA.

US 6255560 (NOTE: USPTO PAIR shows that this patent was allowed to lapse in 3/2005 by owner non-payment of fees. IT IS NO LONGER IN FORCE)
Claim 1A chimeric gene which is expressed in plant cells comprising: a) a promoter from cauliflower mosaic virus (CaMV), wherein said promoter is the CaMV(19S) promoter or the CaMV(35S) promoter, operably linked to a DNA sequence which is heterologous with respect to the promoter, wherein: the promoter regulates the transcription of the DNA sequence, and the DNA sequence encodes a polypeptide conferring increased antibiotic resistance to a plant or plant cell containing the DNA sequence relative to a w ild-type plant or plant cell
Claim 3A plant cell comprising a chimeric gene which comprises: a) a promoter from cauliflower mosaic virus (CaMV), wherein said promoter is the CaMV(19S) promoter or the CaMV(35S) promoter, operably linked to a DNA sequence which is heterologous with respect to the promoter, wherein: the promoter regulates the transcription of the DNA sequence, and the DNA sequence encodes a polypeptide conferring increased antibiotic resistance to the plant cell relative to a wild-type plant cell.
Claim 6An intermediate plant transformation plasmid which comprises: a) a region of homology to an A. tumefaciens vector; b) a T-DNA border from A. tumefaciens, and c) a chimeric gene, wherein the chimeric gene is located between the T-DNA border and the region of homology, said chimeric gene comprising a promoter from cauliflower mosaic virus (CaMV), wherein said promoter is the CaMV(19S) promoter or the CaMV(35S) promoter, operably linked to a DNA sequence which is heterologous with respect to the promoter, wherein: a) the promoter regulates the transcription of the DNA sequence, and b) the DNA sequence encodes a polypeptide conferring increased antibiotic resistance to a plant or plant cell containing the DNA sequence relative to a wild-type plant or plant cell.
Claim 9A plant transformation vector which comprises a modified plant tumor inducing plasmid of A. tumefaciens which is capable of inserting a chimeric gene into susceptible plant cells, wherein the chimeric gene comprises a promoter from cauliflower mosaic virus (CaMV), wherein said promoter is the CaMV(19S) promoter or the CaMV(35S) promoter, operably linked to a DNA sequence which is heterologous with respect to the promoter, wherein: a) the promoter regulates the transcription of the DNA sequence, and b) the DNA sequence encodes a polypeptide conferring increased antibiotic resistance to a plant or plant cell containing the DNA sequence relative to a wild-type plant or plant cell.
Claim 12A differentiated dicotyledonous plant comprising plant cells containing a chimeric gene which comprises a promoter from cauliflower mosaic virus (CaMV), wherein said promoter is the CaMV(19S) promoter or the CaMV(35S) promoter, operably linked to a DNA sequence encoding said polypeptide which is heterologous with respect to the promoter, wherein: a) the promoter regulates the transcription of the DNA sequence, and b) the DNA sequence encodes a polypeptide conferring increased antibiotic resistance to the plant relative to a wild-type plant.

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Claims of Patents granted to Monsanto