US 4960704

Modified hygromycin B resistance gene

Patents granted to Eli Lilly Co. Actual granted independent claims, This patent was assigned to Novartis

US 4960704

Claim 1A DNA sequence which encodes the amino acid sequence:
[sequence] wherein MET is methionine, LYS is lysine, PRO is proline, GLU is glutamic acid, LEU is leucine, THR is threonine, ALA is alanine, SER is serine, VAL is valine, PHE is phenylalanine, ILE is isoleucine, GLY is glycine, ASP is aspartic acid, GLN is glutamine, ARG is arginine, CYS is cysteine, TRP is tryptophan, ASN is asparagine, HIS is histidine and TYR is tyrosine, and R is a dexoxyribunocleotide triplet that encodes lysine and z=0 to 2;
subject to the limitation that said DNA encodes a hygromycin B phosphotransferase that is not encoded by plasmid pKC203.
Claim 30The plasmid pIT20711 This plasmid contains the yeast heat shock gene (YG101) and the truncated hpt gene.
Claim 46A plasmid selected from the group consisting of plasmids pIT141, pIT143, pIT212, pIT213, pIT215, pIT217, and pIT21922 These plasmids contain either the transcriptional and activator sequences of the phosphoglycerate kinase gene (PGK) or of the yeast heat shock gene (YG101) fused to the truncated hpt gene.