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US 5668298

Selectable marker for development of vectors and transformation systems in plants.

Patents granted to Eli Lilly Co. Actual granted independent claims, This patent was assigned to Novartis

US 5668298
Claim 1A chimeric gene functional in a plant cell, which chimeric gene comprises: a) at a position 5′ to coding region (c), a plant-expressible promoter sequence;
b) at a position 3′ to coding region (c), a terminator signal sequence; and
c) a coding region of an aphIV gene, which coding region:

  1. encodes a functional hygromycin phosphotransferase enzyme; and
  2. is positioned between such plant-expressible promoter sequence (a) and such terminator signal sequence (b) so as to be expressible,
    wherein expression of such coding region in a plant cell confers resistance to hygromycin B on such plant cell and
    wherein such resistance to hygromycin B is capable of providing a basis for selection of such plant cell.
Claim 15Plasmid pCEL40 shown in Fig. 2 and derivable by ligating the Bgl II digest of plasmid pCEL30, to the about 1.3 kb BamH I-Bgl II fragment of plasmid pOW20, such plasmids pCEL30 and pOW20 being obtainable from NRRL B-15915 and NRRL B-15838, respectively.