AU 582653 B

Actual granted independent claims

AU 582653 B
Claim 1A DNA sequence having one or two different structural genes and associated control sequence which conveys resistance to either or both of antibiotics hygromycin B and G418 and which is: a) the 7.5 kb Bgl II restriction fragment, b) the 2.75 kb Bgl II/Sal I restriction fragment, c) the 1.51 kb Sac I/Bgl II restriction fragment, or d) the 1.65 kb Eco RI/Sal I restriction fragment of plasmid pKC203.
Claim 5A DNA sequence having one or two different structural genes and associated control sequence which conveys resistance to either or both of antibiotics hygromycin B and G418 substantially as hereinbefore described with particular reference to Examples 2 and 4 to 6¹⁴ . ¹⁴ The examples refer to: Ex. 2: the isolation of the 7.5 kb fragment having both antibiotic resistance genes from plasmid pKC203; and Ex. 4 to 6: the isolation of 1.51 Kb and 1.65 kb fragments from the plasmid pKC222 containing the gene for hygromycin resistance and for G418 resistance respectively.
Claim 6A recombinant DNA cloning vector substantially as described with particular reference to Examples 1, 4, and 7 to 11¹⁵. ¹⁵ The examples refer to: Ex. 1: the construction of plasmid pKC203 having the 7.5 kb fragment with both antibiotic resistance genes; Ex. 4: the construction of plasmid pKC222 having the 2.5 kb fragment with both antibiotic resistance genes; Ex. 7: the construction of plasmid pSC701 having the 7.5 kb fragment with both antibiotic resistance genes; Ex. 8: the construction of plasmid pKC257 with a fragment containing a hygromycin resistance gene and the plasmid pKC259 with a fragment containing resistance genes for both hygromycin and G418; and Ex. 9 to 11: the construction of plasmid pKC261, pKC264 and pKC275 with a fragment containing a hygromycin resistance gene.
Claim 7A transformed host cell substantially as hereinbefore described with particular reference to Examples 1, 4, and 7 to 11¹⁶ . ¹⁶ All examples refer to E. coli transformation.