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US 7196245
Patent or Publication No. Title, Independent Claims and Summary Assigne

US 7196245 B2

  • Earliest priority – 18 Sep 2002
  • Filed – 18 Sep 2003
  • Granted – 27 Mar 2007
  • Expected expiry – 16 Mar 2024
 Title – Polynucleotides and polypeptides that confer increased biomass and tolerance to cold, water deprivation and low nitrogen to plants

Claim 1
A transgenic plant transformed with an expression vector comprising a polynucleotide sequence encoding a polypeptide having a conserved domain that has at least 70% sequence identity to the conserved domain of amino acid coordinates 111–164 of SEQ ID NO: 194, wherein the conserved domain is a WRKY DNA-binding domain and expression of the polynucleotide in said transgenic plant results in said plant having a greater tolerance to water deprivation than a control plant.
Claim 4
A method for producing a transgenic plant having greater tolerance to water deprivation than a control plant, the method steps comprising (a) providing an expression vector comprising (i) a polynucleotide sequence encoding a polypeptide comprising a conserved domain that has at least 70% sequence identity to the conserved domain of amino acid coordinates 111–164 of SEQ ID NO: 194, wherein the conserved domain is a WRKY DNA-binding domain and expression of the polynucleotide in said transgenic plant results in said plant having a greater tolerance to water deprivation than a control plant; and(ii) at least one regulatory element operably linked to the polynucleotide sequence, wherein said at least one regulatory element controls expression of the polynucleotide sequence in a target plant;(b) introducing the expression vector into at least one plant; and(c) selecting at least one transgenic plant that has greater tolerance to water deprivation than the control plant.
Claim 12
A method for increasing the tolerance of a plant to water deprivation, the method steps comprising (a) providing an expression vector comprising (i) a polynucleotide sequence encoding a polypeptide comprising a conserved domain that has at least 70% sequence identity to the conserved domain of amino acid coordinates 111–164 of SEQ ID NO: 194, wherein the conserved domain is a WRKY DNA-binding domain and expression of the polynucleotide in the transgenic plant results in said plant having a greater tolerance to water deprivation than a control plant; and(ii) at least one regulatory element flanking the polynucleotide sequence, wherein said at least one regulatory element controls expression of the polynucleotide sequence in a target plant;(b) introducing the expression vector into a plant, thereby producing a transgenic plant; and(c) selecting a transgenic plant having greater tolerance to water deprivation than a control plant.

Mendel Biotechnology Inc.