US 7193129

Patent or Publication No. Title, Independent Claims and Summary Assigne

US 7193129 B2

  • Earliest priority – 18 Apr 2001
  • Filed – 9 Aug 2002
  • Granted – 20 Mar 2007
  • Expected expiry – 24 Jun 2022
Title – Stress-related polynucleotides and polypeptides in plants

Claim 1
A method for increasing the tolerance of a plant to nitrogen limiting conditions as compared to a wild-type plant of the same species, the method steps comprising (a) inserting a recombinant polynucleotide having a nucleotide sequence encoding a polypeptide comprising a conserved domain with at least about 80% sequence identity to a conserved domain of amino acid coordinates 39–76 of SEQ ID NO: 52 into an expression vector, wherein the conserved domain is a DNA-binding domain;(b) introducing the expression vector into a target plant or plant cell to generate at least one transformed plant, wherein the polypeptide is overexpressed in the at least one transformed plant and said overexpression of the polypeptide results in the at least one transformed plant having greater tolerance to the nitrogen limiting conditions as compared to the wild-type plant; and(c) from the at least one transformed plant, identifying a transgenic plant having greater tolerance to the nitrogen limiting conditions than the wild-type plant as a result of the overexpression of the polypeptide in the transgenic plant.
Claim 7
A method for producing a transgenic plant having greater tolerance to nitrogen limiting conditions than a wild-type plant of the same species, the method steps comprising (a) inserting a recombinant polynucleotide having a nucleotide sequence encoding a polypeptide comprising a conserved domain with at least about 80% sequence identity to a conserved domain of amino acid coordinates 39–76 of SEQ ID NO: 52 into an expression vector, wherein the conserved domain is a DNA-binding domain;(b) introducing the expression vector into a target plant or plant cell to generate at least one transformed plant, wherein the polypeptide is overexpressed in the at least one transformed plant and said overexpression of the polypeptide results in the at least one transformed plant having greater tolerance to the nitrogen limiting conditions as compared to the wild-type plant; and(c) from the at least one transformed plant, identifying a transgenic plant having greater tolerance to the nitrogen limiting conditions than the wild-type plant as a result of the overexpression of the polypeptide in the transgenic plant.

Mendel Biotechnology Inc.