Title – Biosynthetic pathway transcription factors
Claim 1
A method of determining whether a member of a pool of cloned test transcription factor polynucleotides encodes a plant pathway transcription factor, the method comprising: collecting a pool of cloned test transcription factor polynucleotides; introducing into a plant cell a nucleic acid comprising a plant promoter of a pathway gene operably linked to a reporter gene; introducing into the plant cell a member of the pool of cloned test transcription factor polynucleotides, wherein said member is selected on the basis of structural similarity to a known transcription factor for a pathway gene; and detecting expression of said reporter gene in the plant cell, thereby determining whether the member of the cloned test transcription factor polynucleotide pool encodes a plant pathway transcription factor.
Claim 15
A method of determining whether a member of a pool of test transcription factor polynucleotides encodes a biosynthetic pathway transcription factor, comprising: PA1 introducing into a plant cell nucleic acids comprising the test transcription factor polynucleotides and detecting expression of a biosynthetic pathway gene in the plant cell by quantitation of the biosynthetic pathway gene RNA level, wherein said member is selected on the basis of structural similarity to a known transcription factor for a pathway gene; PA1 thereby determining whether the member of the cloned test transcription factor polynucleotide pool encodes a plant pathway transcription factor.
Claim 16
A method of determining whether two or more members of a pool of cloned test transcription factor polynucleotides are required for expression from a pathway gene promoter, wherein said two or more members of a pool of cloned test transcription factor polynucleotides are selected on the basis of structural similarity to a known transcription factor for a pathway gene, the method comprising: PA1 collecting a pool of cloned test transcription factor polynucleotides; PA1 introducing into plant cell a nucleic acid comprising a biosynthetic pathway gene promoter operably linked to a reporter gene; PA1 introducing into plant cell the pool of cloned test transcription factor polynucleotides; PA1 detecting expression from said biosynthetic pathway gene promoter in the plant cell, and deconvoluting the pool of cloned test transcription factor polynucleotides to identify the minimum number of cloned test transcription factor polynucleotides necessary to detect expression from said biosynthetic pathway gene promoter; PA1 thereby determining whether two or more members of the cloned test transcription factor polynucleotide pool are required for expression from said biosynthetic pathway gene promoter.
