Arabidopsis thaliana Genomic Sequencing
The Arabidopsis Genome Initiative (AGI) was set up in 1996. Consisting of 6 groups, it’s mission was to sequence the genome of Arabidopsis thaliana by 2004 at high accuracy. Various aspects of the sequencing mission were divided between the 6 groups:
 |
Links: |
The sequencing project was completed in 2000, four years ahead of schedule.
The sequence derived from the AGI’s efforts were the subject of a number of publications in nature, including:
- Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana (Nature. 1999 Dec 16;402(6763):761-8)
- Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana (Nature. 1999 Dec 16;402(6763):769-77)
- Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana (Nature. 2000 Dec 14;408(6814):816-20)
- Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana (Nature. 2000 Dec 14;408(6814):820-2)
- Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana (Nature. 2000 Dec 14;408(6814):823-6)
- Analysis of the genome sequence of the flowering plant Arabidopsis thaliana (Nature, 408:796-815).
Before, during, and subsequent to the AGI’s efforts, groups, including some of those involved in the genomic sequencing, were involved in sequencing Expressed Sequence Tags (ESTs) from Arabidopsis. The following companies and institutes were involved in these efforts:
- Ceres Inc (~5000 ESTs deposited with TIGR)
- Kazusa DNA Research Institute, 1532-3 Yana, Kisarazu, Chiba 292-0812, Japan (12,028 non-redundant ESTs)
- TIGR
- Cold Spring Harbor Laboratory
- Washington University School of Medicine, Genome Sequencing Center
- Laboratoire de Biologie Cellulaire, INRA, Versailles Cedex, France/CNRS/ consortia (Plant J. 1993 Dec;4(6):1051-61; Plant J. 1996 Jan;9(1):101-24)
- Department of Energy Plant Research Laboratory, Michigan State University and Consortia (Plant Physiol. 2000 Dec;124(4):1582-94)
Today there are more than 622,000 Arabidopsis ESTs in the dbEST database at the National Center for Biotechnology Information (NCBI).