35S-enhanced mas promoter – Specific Patent Information

Patent Number Title, Independent Claims and Summary of Claims Assignee
US 5106739

  • Earliest priority – 18 April 1989
  • Filed – 7 February 1990
  • Granted – 21 April 1992
  • Expected expiry – 21 April 2009
Title – CaMV 35S-enhanced mannopine synthase promoter and method for using same

Claim 1
A DNA sequence comprising, in the 5′ to 3′ direction, a first element linked to a second element, said first element comprising an upstream activating region of CaMV 35S and said second element comprising a mannopine synthase transcription initiation region.
Claim 9
A chimeric promoter comprising a CaMV 35S enhanced mannopine synthase promoter, wherein upon expression of a DNA sequence of interest in a plant cell under the regulatory control of said promoter, said DNA sequence of interest is expressible at a level of at least 5-fold higher than expression of said gene of interest in a plant cell under the regulatory control of a CaMV 35S enhanced CaMV 35S promoter.
Claim 16
A method to increase the expression of an expressible gene of interest under the regulatory control of a mannopine synthase promotercomprising the steps of:
providing a CaMV 35S upstream activating region to the 5′ end of a DNA sequence comprising the mannopine synthase promoter; and
allowing said gene to be expressed.

The claims are generally directed to:

  • A DNA sequence comprising (in 5′ to 3′ direction): an UAR of CaMV 35S and a mas transcription initiation region.
  • A chimeric CaMV 35S-enhanced mas promoter  regulating the expression of a gene of interest in a plant. The expression level is at least 5-fold higher than the expression level achieved under the control of a CaMV 35S-enhanced 35S promoter.
  • A method to increase the expression of a gene of interest by using the described chimeric promoter.

Originally assigned to Calgene

(now owned byMonsanto)

CA 1334175

  • Earliest priority – 18 April 1989
  • Filed – 29 September 1989
  • Granted – 31 January 1995
  • Expected  expiry – 30 January 2012
Title – Plant promoter and method for using same

The independent claims are the same as US 5106739.

Note: Patent information was last updated on 28 February 2006. Search terms: “CaMV” in abstract and “Calgene” in applicant. Patent database: PatentLens in combination with INPADOC.