Pioneer Hi-Bred had filed patent applications related to plant promoters containing elements that drive the expression of genes of interest in root cells and tissues.
The claims as filed of the patent applications also describe very broad methods for the isolation and characterization of tissue-specific plant promoters in general. If the claims as filed are granted without change they would potentially cover processes for the identification of tissue-specific promoters regardless of the tissue where the expression of nucleotide sequences is sought.
Pioneer Hi-Bred has now obviously abandoned this patent, although it is still pending in Australia.
Pioneer Hi-Bred‘s patent applications in Australia, the U.S. and Europe cover the following aspects:
- Methods for identifying and isolating tissue-preferred plant promoter elements in general.
- The elements are not restricted to any particular tissue in the claims as filed.
- Plant promoters containing root-preferred promoter elements that enhance or suppress the expression of a linked sequence in root cells.
- Specific sequences of root-promoter elements are spelled out in the claims as filed. Some plant promoters of the invention have multiple root-preferred promoter elements.
- Methods for root-preferred expression of genes in plants by transforming a plant with an expression cassette having a promoter with elements for root expression; and
- Plant cells stably transformed with DNA construct containing root-preferred expression elements.
The term “root-preferred” means that the expression driven by a plant promoter of the invention is selectively enhanced or suppressed in roots in comparison to other tissues. Root cells and tissues include any part of the roots, and cover primary, lateral and adventitious roots.
Plants to be transformed with the constructs of the invention are not limited to any in particular in the claims as filed.
|Patent No||Title, Independent Claims and Summary||Applicant|
||Title – Novel root-preferred promoter elements and method of use
|Related application in the United States (US 2001/047525 A1) has been expressly abandoned. Applications in Europe (EP 1248850 A2) and Canada (CA 2390819) are also lapsed.|