Menu

About Us

Cambia is an independent non-profit institute creating new technologies, tools and paradigms to promote change and enable innovation.

Read More

Patents on 35S promoter
Patent Number
 Title,  Independent Claims and Summary of Claims Assignee
US 5352605

  • Earliest priority – 17 January 1983
  • Filed – 28 October 1993
  • Granted – 4 October 1994
  • Expected expiry – 4 October 2011
 Title – Chimeric genes for transforming plant cells using viral promoters

Claim 1
A chimeric gene which is expressed in plant cells comprising a promoter from a cauliflower mosaic virus, said promoter selected from the group consisting of a CaMV (35S) promoter isolated from CaMV protein-encoding DNA sequences and a CaMV (19S) promoter isolated from CaMV protein-encoding DNA sequences, and a structural sequence which is heterologous with respect to the promoter.
Claim 4
A plant cell which comprises a chimeric gene that contains a promoter from cauliflower mosaic virus, said promoter selected from the group consisting of a CaMV (35S) promoter and a CaMV (19S) promoter, wherein said promoter is isolated from CaMV protein-encoding DNA sequences, and a structural sequence which is heterologous with respect to the promoter.
Claim 7
An intermediate plant transformation plasmid which comprises a region of homology to an Agrobacterium tumefaciens vector, a T-DNA border region from Agrobacterium tumefaciens and a chimeric gene, wherein the chimeric gene is located between the T-DNA border and the region of homology, said chimeric gene comprising a promoter from cauliflower mosaic virus, said promoter selected from the group consisting of a CaMV(35S) promoter and a CaMV(19S) promoter, and a structural sequence which is heterologous with respect to the promoter.
Claim 8
A plant transformation vector which comprises a disarmed plant tumor inducing plasmid of Agrobacterium tumefaciens and a chimeric gene, wherein the chimeric gene contains a promoter from cauliflower mosaic virus, said promoter selected from the group consisting of a CaMV(35S) promoter and a CaMV(19S) promoter, and a structural sequence which is heterologous with respect to the promoter.
Claim 13
A DNA construct comprising: (A) a CaMV promoter selected from the group consisting of (1) a CaMV 35S promoter isolated from CaMV protein-encoding DNA sequences and (2) a CaMV 19S promoter isolated from CaMV protein-encoding DNA sequences, and (B) a DNA sequence of interest heterologous to (A), wherein (B) is under the regulatory control of (A) when said construct is transcribed in a plant cell.
Claim 14
A chimeric gene which is transcribed and translated in plant cells, said chimeric gene comprising a promoter from cauliflower mosaic virus, said promoter selected from the group consisting of: a) a CaMV 35S promoter region free of CaMV protein-encoding DNA sequences and b) a CaMV 19S promoter region free of CaMV protein-encoding DNA sequences, and a DNA sequence which is heterologous with respect to the promoter.
Claim 15
A chimeric gene which is expressed in plants cells comprising a promoter from a cauliflower mosaic virus, said promoter selected from the group consisting of a CaMV(35S) promoter region free of CaMV protein-encoding DNA sequences and a CaMV(19S) promoter region free of CaMV protein-encoding DNA sequences, and a DNA sequence which is heterologous with respect to the promoter.
Claim 16
A chimeric gene which is transcribed in plants cells comprising a promoter from a cauliflower mosaic virus, said promoter selected from the group consisting of a CaMV(35S) promoter free of CaMV protein-encoding DNA sequences and a CaMV(19S) promoter free of CaMV protein-encoding DNA sequences, a DNA sequence which is heterologous with respect to the promoter and a 3′ non-translated polyadenylation signal sequence.
Claim 17
A plant cell which comprises a chimeric gene where said chimeric gene comprises a promoter from cauliflower mosaic virus, said promoter selected from the group consisting of a CaMV(35S) promoter and a CaMV(19S) promoter, wherein said promoter is free of CaMV protein-encoding DNA sequences, and a DNA sequence which is heterologous with respect to the promoter and a 3′ non-translated polyadenylation signal sequence.

Claim elements:

  • 35S and 19S promoters, as defined in the specification, are claimed as part of a chimeric construct, also containing a heterologous signal and a poly(A) signal, expressed in a plant
  • There are also claims to intermediate plant transformation vectors and plant transformation vectors containing the chimeric gene forAgrobacterium-mediated plant transformation, and to plant cells having the chimeric gene.
 Monsanto
US 5530196

  • Earliest priority – 17 January 1983
  • Filed – 2 September 1994
  • Granted – 25 June 1996
  • Expected expiry – 25 June 2013
 Title – Chimeric genes for transforming plant cells using viral promoters

Claim 1
A differentiated dicotyledonous plant comprising plant cells containing a chimeric gene which comprises a promoter from cauliflower mosaic virus, said promoter selected from the group consisting of a CaMV(35S) promoter free of CaMV protein-encoding DNA sequences and a CaMV(19S) promoter free of protein-encoding DNA sequences, and a structural sequence which is heterologous with respect to the promoter.
Claim 4
A differentiated dicotyledonous plant comprising plant cells containing in the plant genome a chimeric gene which comprises a promoter from cauliflower mosaic virus, said promoter selected from the group consisting of a CaMV(35S) promoter and a CaMV(19S) promoter, and a DNA sequence which is heterologous with respect to the promoter.
Claim 5
A differentiated dicotyledonous plant regenerated from plant cells, said plant cells containing a chimeric gene which comprises a promoter from cauliflower mosaic virus, said promoter selected from the group consisting of a CaMV(35S) promoter and a CaMV(19S) promoter, and a DNA sequence which is heterologous with respect to the promoter.

This patent is a Continuation of US 5352605.

  • Differentiated dicot plants having plant cells with a chimeric gene similar as described above. The poly(A) signal is not expressly claimed as a component of the chimeric gene.
US 5858742

  • Earliest priority – 17 January 1983
  • Filed – 24 June 1996
  • Granted – 12 January 1999
  • Expected expiry – 25 June 2013
 Title – Chimeric genes for transforming plant cells using viral promoters

Claim 1
A method for transforming a plant cell which comprises transforming a plant cell with a chimeric DNA construct containing a promoter isolated from cauliflower mosaic virus (CaMV), said promoter selected from the group consisting of a CaMV(19S) promoter derived from the CaMV(19S) gene and a CaMV(35S) promoter derived from the CaMV(35S) gene, and a DNA sequence which is heterologous with respect to the promoter; wherein the promoter regulates the transcription of the DNA sequence.

This patent is a Continuation of US 5530196.

  • Method for transforming plant cells with a chimeric DNA construct as generally described in US 5530196.
US 6255560

  • Earliest priority – 17 January 1983
  • Filed – 11 January 1999
  • Granted – 3 July 2001
  • Expired – 3 August 2005
 Title – Chimeric genes for transforming plant cells using viral promoters

Claim 1
A chimeric gene which is expressed in plant cells comprising a promoter from cauliflower mosaic virus (CaMV), wherein said promoter is the CaMV(19S) promoter or the CaMV(35S) promoter, operably linked to a DNA sequence which is heterologous with respect to the promoter, wherein: the promoter regulates the transcription of the DNA sequence, and the DNA sequence encodes a polypeptide conferring increased antibiotic resistance to a plant or plant cell containing the DNA sequence relative to a wild-type plant or plant cell.
Claim 3
A plant cell comprising a chimeric gene which comprises a promoter from cauliflower mosaic virus (CaMV), wherein said promoter is the CaMV(19S) promoter or the CaMV(35S) promoter, operably linked to a DNA sequence which is heterologous with respect to the promoter, wherein: the promoter regulates the transcription of the DNA sequence, and the DNA sequence encodes a polypeptide conferring increased antibiotic resistance to the plant cell relative to a wild-type plant cell.
Claim 6
An intermediate plant transformation plasmid which comprises a region of homology to an Agrobacterium tumefaciens vector, a T-DNA border from Agrobacterium tumefaciens, and a chimeric gene, wherein the chimeric gene is located between the T-DNA border and the region of homology, said chimeric gene comprising a promoter from cauliflower mosaic virus (CaMV), wherein said promoter is the CaMV(19S) promoter or the CaMV(35S) promoter, operably linked to a DNA sequence which is heterologous with respect to the promoter, wherein: the promoter regulates the transcription of the DNA sequence, and the DNA sequence encodes a polypeptide conferring increased antibiotic resistance to a plant or plant cell containing the DNA sequence relative to a wild-type plant or plant cell.
Claim 9
A plant transformation vector which comprises a modified plant tumor inducing plasmid of Agrobacterium tumefaciens which is capable of inserting a chimeric gene into susceptible plant cells, wherein the chimeric gene comprises a promoter from cauliflower mosaic virus (CaMV), wherein said promoter is the CaMV(19S) promoter or the CaMV(35S) promoter, operably linked to a DNA sequence which is heterologous with respect to the promoter, wherein: the promoter regulates the transcription of the DNA sequence, and the DNA sequence encodes a polypeptide conferring increased antibiotic resistance to a plant or plant cell containing the DNA sequence relative to a wild-type plant or plant cell.
Claim 12
A differentiated dicotyledonous plant comprising plant cells containing a chimeric gene which comprises a promoter from cauliflower mosaic virus (CaMV), wherein said promoter is the CaMV(19S) promoter or the CaMV(35S) promoter, operably linked to a DNA sequence encoding said polypeptide which is heterologous with respect to the promoter, wherein: the promoter regulates the transcription of the DNA sequence, and the DNA sequence encodes a polypeptide conferring increased antibiotic resistance to the plant relative to a wild-type plant.

This patent is a Continuation of US 5858742.

  • A chimeric gene where the 35S or the 19S promoter regulates the transcription of a gene conferring antibiotic resistance to a plant.
  • An intermediate plant transformation vector with the mentioned chimeric gene between a T-DNA border and a region of homology to an Agrobacterium tumefaciens vector.
  • A plant transformation vector capable of inserting the chimeric gene into a plant cells. Differentiated dicot plants having plant cells with the chimeric gene.
EP 131623 B2

  • Earliest priority – 17 January 1983
  • Filed – 16 January 1984
  • Granted – 28 July 1999
  • Lapsed after termination of 20 years
 Title – Chimeric genes suitable for expression in plant cells

Claim1
A chimeric gene capable of expressing a neomycin phospotransferase polypeptide in plant cells conferring antibiotic resistance to the plant when inserted into the plant genome, comprising in sequence:a) a promoter region from a ribulose-1,5-bis-phosphate carboxyase small subunit gene;
b) a 5′ non-translated region
c) a structural coding sequence encoding neomycin phospotransferase I or II; and
d) a 3′ non-translated region of a gene naturally expressed in plant cells, said region encoding a signal sequence for polyadenylation of mRNA, said promoter region being heterologous with respect to the structural coding sequence .
Claim 4
A chimeric gene capable of expressing a polypeptide in plant cells comprising in sequence:a) a full-length transcript promoter region isolated from cauliflower mosaic virus
b) a 5′ non-translated region
c) a structural coding sequence
d) a 3′ non-translated region of a gene naturally expressed in plants, said region encoding a signal sequence for polyadenylation of mRNA, said structural coding sequence being heterologous with respect to said promoter region.
Claim 6
A culture of microorganisms identified by ATCC accession number 39265.

The claims are to:

  • A chimeric gene capable of expressing a polypeptide in plant cells having a 35S promoter, an intron, a structural coding sequence and a poly(A) signal.

Remarks

 Granted Japanese patent JP 2645217 was not analyzed. There are also a pending Japanese application (JP 7014349 B4) and a pending Brazilian application (BR 1101069).

Note: Patent information on this page was last updated on 15 March 2006.