Patents on 35S promoter

Patent Number
Title,  Independent Claims and Summary of Claims Assignee
US 5352605

  • Earliest priority – 17 January 1983
  • Filed – 28 October 1993
  • Granted – 4 October 1994
  • Expected expiry – 4 October 2011
Title – Chimeric genes for transforming plant cells using viral promoters

Claim 1
A chimeric gene which is expressed in plant cells comprising a promoter from a cauliflower mosaic virus, said promoter selected from the group consisting of a CaMV (35S) promoter isolated from CaMV protein-encoding DNA sequences and a CaMV (19S) promoter isolated from CaMV protein-encoding DNA sequences, and a structural sequence which is heterologous with respect to the promoter.
Claim 4
A plant cell which comprises a chimeric gene that contains a promoter from cauliflower mosaic virus, said promoter selected from the group consisting of a CaMV (35S) promoter and a CaMV (19S) promoter, wherein said promoter is isolated from CaMV protein-encoding DNA sequences, and a structural sequence which is heterologous with respect to the promoter.
Claim 7
An intermediate plant transformation plasmid which comprises a region of homology to an Agrobacterium tumefaciens vector, a T-DNA border region from Agrobacterium tumefaciens and a chimeric gene, wherein the chimeric gene is located between the T-DNA border and the region of homology, said chimeric gene comprising a promoter from cauliflower mosaic virus, said promoter selected from the group consisting of a CaMV(35S) promoter and a CaMV(19S) promoter, and a structural sequence which is heterologous with respect to the promoter.
Claim 8
A plant transformation vector which comprises a disarmed plant tumor inducing plasmid of Agrobacterium tumefaciens and a chimeric gene, wherein the chimeric gene contains a promoter from cauliflower mosaic virus, said promoter selected from the group consisting of a CaMV(35S) promoter and a CaMV(19S) promoter, and a structural sequence which is heterologous with respect to the promoter.
Claim 13
A DNA construct comprising: (A) a CaMV promoter selected from the group consisting of (1) a CaMV 35S promoter isolated from CaMV protein-encoding DNA sequences and (2) a CaMV 19S promoter isolated from CaMV protein-encoding DNA sequences, and (B) a DNA sequence of interest heterologous to (A), wherein (B) is under the regulatory control of (A) when said construct is transcribed in a plant cell.
Claim 14
A chimeric gene which is transcribed and translated in plant cells, said chimeric gene comprising a promoter from cauliflower mosaic virus, said promoter selected from the group consisting of: a) a CaMV 35S promoter region free of CaMV protein-encoding DNA sequences and b) a CaMV 19S promoter region free of CaMV protein-encoding DNA sequences, and a DNA sequence which is heterologous with respect to the promoter.
Claim 15
A chimeric gene which is expressed in plants cells comprising a promoter from a cauliflower mosaic virus, said promoter selected from the group consisting of a CaMV(35S) promoter region free of CaMV protein-encoding DNA sequences and a CaMV(19S) promoter region free of CaMV protein-encoding DNA sequences, and a DNA sequence which is heterologous with respect to the promoter.
Claim 16
A chimeric gene which is transcribed in plants cells comprising a promoter from a cauliflower mosaic virus, said promoter selected from the group consisting of a CaMV(35S) promoter free of CaMV protein-encoding DNA sequences and a CaMV(19S) promoter free of CaMV protein-encoding DNA sequences, a DNA sequence which is heterologous with respect to the promoter and a 3′ non-translated polyadenylation signal sequence.
Claim 17
A plant cell which comprises a chimeric gene where said chimeric gene comprises a promoter from cauliflower mosaic virus, said promoter selected from the group consisting of a CaMV(35S) promoter and a CaMV(19S) promoter, wherein said promoter is free of CaMV protein-encoding DNA sequences, and a DNA sequence which is heterologous with respect to the promoter and a 3′ non-translated polyadenylation signal sequence.

Claim elements:

  • 35S and 19S promoters, as defined in the specification, are claimed as part of a chimeric construct, also containing a heterologous signal and a poly(A) signal, expressed in a plant
  • There are also claims to intermediate plant transformation vectors and plant transformation vectors containing the chimeric gene forAgrobacterium-mediated plant transformation, and to plant cells having the chimeric gene.
Monsanto
US 5530196

  • Earliest priority – 17 January 1983
  • Filed – 2 September 1994
  • Granted – 25 June 1996
  • Expected expiry – 25 June 2013
Title – Chimeric genes for transforming plant cells using viral promoters

Claim 1
A differentiated dicotyledonous plant comprising plant cells containing a chimeric gene which comprises a promoter from cauliflower mosaic virus, said promoter selected from the group consisting of a CaMV(35S) promoter free of CaMV protein-encoding DNA sequences and a CaMV(19S) promoter free of protein-encoding DNA sequences, and a structural sequence which is heterologous with respect to the promoter.
Claim 4
A differentiated dicotyledonous plant comprising plant cells containing in the plant genome a chimeric gene which comprises a promoter from cauliflower mosaic virus, said promoter selected from the group consisting of a CaMV(35S) promoter and a CaMV(19S) promoter, and a DNA sequence which is heterologous with respect to the promoter.
Claim 5
A differentiated dicotyledonous plant regenerated from plant cells, said plant cells containing a chimeric gene which comprises a promoter from cauliflower mosaic virus, said promoter selected from the group consisting of a CaMV(35S) promoter and a CaMV(19S) promoter, and a DNA sequence which is heterologous with respect to the promoter.

This patent is a Continuation of US 5352605.

  • Differentiated dicot plants having plant cells with a chimeric gene similar as described above. The poly(A) signal is not expressly claimed as a component of the chimeric gene.
US 5858742

  • Earliest priority – 17 January 1983
  • Filed – 24 June 1996
  • Granted – 12 January 1999
  • Expected expiry – 25 June 2013
Title – Chimeric genes for transforming plant cells using viral promoters

Claim 1
A method for transforming a plant cell which comprises transforming a plant cell with a chimeric DNA construct containing a promoter isolated from cauliflower mosaic virus (CaMV), said promoter selected from the group consisting of a CaMV(19S) promoter derived from the CaMV(19S) gene and a CaMV(35S) promoter derived from the CaMV(35S) gene, and a DNA sequence which is heterologous with respect to the promoter; wherein the promoter regulates the transcription of the DNA sequence.

This patent is a Continuation of US 5530196.

  • Method for transforming plant cells with a chimeric DNA construct as generally described in US 5530196.
US 6255560

  • Earliest priority – 17 January 1983
  • Filed – 11 January 1999
  • Granted – 3 July 2001
  • Expired – 3 August 2005
Title – Chimeric genes for transforming plant cells using viral promoters

Claim 1
A chimeric gene which is expressed in plant cells comprising a promoter from cauliflower mosaic virus (CaMV), wherein said promoter is the CaMV(19S) promoter or the CaMV(35S) promoter, operably linked to a DNA sequence which is heterologous with respect to the promoter, wherein: the promoter regulates the transcription of the DNA sequence, and the DNA sequence encodes a polypeptide conferring increased antibiotic resistance to a plant or plant cell containing the DNA sequence relative to a wild-type plant or plant cell.
Claim 3
A plant cell comprising a chimeric gene which comprises a promoter from cauliflower mosaic virus (CaMV), wherein said promoter is the CaMV(19S) promoter or the CaMV(35S) promoter, operably linked to a DNA sequence which is heterologous with respect to the promoter, wherein: the promoter regulates the transcription of the DNA sequence, and the DNA sequence encodes a polypeptide conferring increased antibiotic resistance to the plant cell relative to a wild-type plant cell.
Claim 6
An intermediate plant transformation plasmid which comprises a region of homology to an Agrobacterium tumefaciens vector, a T-DNA border from Agrobacterium tumefaciens, and a chimeric gene, wherein the chimeric gene is located between the T-DNA border and the region of homology, said chimeric gene comprising a promoter from cauliflower mosaic virus (CaMV), wherein said promoter is the CaMV(19S) promoter or the CaMV(35S) promoter, operably linked to a DNA sequence which is heterologous with respect to the promoter, wherein: the promoter regulates the transcription of the DNA sequence, and the DNA sequence encodes a polypeptide conferring increased antibiotic resistance to a plant or plant cell containing the DNA sequence relative to a wild-type plant or plant cell.
Claim 9
A plant transformation vector which comprises a modified plant tumor inducing plasmid of Agrobacterium tumefaciens which is capable of inserting a chimeric gene into susceptible plant cells, wherein the chimeric gene comprises a promoter from cauliflower mosaic virus (CaMV), wherein said promoter is the CaMV(19S) promoter or the CaMV(35S) promoter, operably linked to a DNA sequence which is heterologous with respect to the promoter, wherein: the promoter regulates the transcription of the DNA sequence, and the DNA sequence encodes a polypeptide conferring increased antibiotic resistance to a plant or plant cell containing the DNA sequence relative to a wild-type plant or plant cell.
Claim 12
A differentiated dicotyledonous plant comprising plant cells containing a chimeric gene which comprises a promoter from cauliflower mosaic virus (CaMV), wherein said promoter is the CaMV(19S) promoter or the CaMV(35S) promoter, operably linked to a DNA sequence encoding said polypeptide which is heterologous with respect to the promoter, wherein: the promoter regulates the transcription of the DNA sequence, and the DNA sequence encodes a polypeptide conferring increased antibiotic resistance to the plant relative to a wild-type plant.

This patent is a Continuation of US 5858742.

  • A chimeric gene where the 35S or the 19S promoter regulates the transcription of a gene conferring antibiotic resistance to a plant.
  • An intermediate plant transformation vector with the mentioned chimeric gene between a T-DNA border and a region of homology to an Agrobacterium tumefaciens vector.
  • A plant transformation vector capable of inserting the chimeric gene into a plant cells. Differentiated dicot plants having plant cells with the chimeric gene.
EP 131623 B2

  • Earliest priority – 17 January 1983
  • Filed – 16 January 1984
  • Granted – 28 July 1999
  • Lapsed after termination of 20 years
Title – Chimeric genes suitable for expression in plant cells

Claim1
A chimeric gene capable of expressing a neomycin phospotransferase polypeptide in plant cells conferring antibiotic resistance to the plant when inserted into the plant genome, comprising in sequence:a) a promoter region from a ribulose-1,5-bis-phosphate carboxyase small subunit gene;
b) a 5′ non-translated region
c) a structural coding sequence encoding neomycin phospotransferase I or II; and
d) a 3′ non-translated region of a gene naturally expressed in plant cells, said region encoding a signal sequence for polyadenylation of mRNA, said promoter region being heterologous with respect to the structural coding sequence .
Claim 4
A chimeric gene capable of expressing a polypeptide in plant cells comprising in sequence:a) a full-length transcript promoter region isolated from cauliflower mosaic virus
b) a 5′ non-translated region
c) a structural coding sequence
d) a 3′ non-translated region of a gene naturally expressed in plants, said region encoding a signal sequence for polyadenylation of mRNA, said structural coding sequence being heterologous with respect to said promoter region.
Claim 6
A culture of microorganisms identified by ATCC accession number 39265.

The claims are to:

  • A chimeric gene capable of expressing a polypeptide in plant cells having a 35S promoter, an intron, a structural coding sequence and a poly(A) signal.

Remarks

Granted Japanese patent JP 2645217 was not analyzed. There are also a pending Japanese application (JP 7014349 B4) and a pending Brazilian application (BR 1101069).

Note: Patent information on this page was last updated on 15 March 2006.