Ubiquitin promoters from rice and sugarcane

Apart from patents on the ubiquitin regulatory system from maize, there are also patents specifically claiming ubiquitin promoters from rice and sugarcane.

The Board of Supervisors of Louisiana State University and Agricultural and Mechanical College holds a United States patent (US 6528701) on rice ubiquitin promoters. This patent claims a rice ubiquitin promoter and a method for using it in controlling gene expression in plants. It shows in the description that the rice ubiquitin promoter is stronger than the maize ubiquitin promoter in driving gene expression in rice.

United States of America as represented by the Secretory of Agriculture & University of Hawaii has three United States patents (US 6706948US 6686513 and US 6638766) on sugarcane ubiquitin promoters (UBI4 and UBI9).

rice ubiquitin promoters

Title, Independent Claims and Summary of Claims

Assignee

US 6528701

  • Earliest priority – 29 Feb 2000
  • Filed – 29 Feb 2000
  • Granted – 04 Mar 2003
  • Expected expiry – 29 Feb 2020
Title – Rice ubiquitin-derived promoters

Claim 1
An isolated nucleotide sequence comprising a rice ubiquitin promoter capable of controlling constitutive expression of a nucleic acid encoding a polypeptide, wherein said nucleotide sequence comprises at least a portion of SEQ ID NO:3 which is upstream of position 2785 and wherein said portion retains promoter activity.

The claims of this patent are generally drawn to:

  • An isolated nucleotide sequence comprising a rice ubiquitin promoter (upstream of position 2785 of SEQ ID NO:3) , and
  • A method of expressing a heterologous nucleic acid sequence encoding a polypeptide in a plant or in its progeny under the control of the rice ubiquitin promoter (claim 6).

Board of Supervisors of Louisiana State University and Agricultural and Mechanical College

Note: Patent information was last updated on 31 October 2006. Search terms: “Ubiquitin promoter” in abstract. Patent database: PatentLens in combination with INPADOC.

Patents and applications on sugarcane ubiquitine promoters

Patent/application number

Title, Independent Claims and Summary of Claims

Assignee

US 6706948

  • Earliest priority – 19 Mar 1998
  • Filed – 17 Mar 1999
  • Granted – 16 Mar 2004
  • Expected expiry – 17 Mar  2019
Title –  Sugarcane UBI9 gene promoter and methods of use thereof

Claim 1
A substantially purified nucleic acid sequence comprising a nucleotide sequence selected from the group consisting of SEQ ID NO:7, the complement of SEQ ID NO:7SEQ ID NO:10, and the complement of SEQ ID NO:10.
Claim 4
A substantially purified nucleic acid sequence comprising the HindIII/XbaI fragment isolated from plasmid pubi9-GUS contained in Escherichia coli cells deposited as NRRLB-30116.
Claim 5
A transgenic plant cell comprising a nucleic acid sequence comprising a double-stranded nucleotide sequence listed as SEQ ID NO:10, wherein said nucleotide sequence is operably linked to a nucleic acid sequence of interest.
Claim 6
A method for expressing a nucleic acid sequence of interest in a plant cell, comprising:a) providing:

i) a plant cell;

ii) a nucleic acid sequence of interest; and

iii) a nucleotide sequence selected from the group consisting of SEQ ID NO:10, and the complement ofSEQ ID NO:10;

b) operably linking said nucleic acid sequence of interest to said nucleotide sequence to produce a transgene; and

c) introducing said transgene into said plant cell to produce a transgenic plant cell under conditions such that said nucleic acid sequence of interest is expressed in said transgenic plant cell.

Claim 7
A method for expressing a nucleic acid sequence of interest in a plant cell, comprising:a) providing:

i) a plant cell;

ii) a nucleic acid sequence of interest; and

iii) a promoter comprising SEQ ID NO:10;

b) operably linking said nucleic acid sequence of interest to said promoter to produce a transgene;

c) introducing said transgene into said plant cell to produce a transgenic plant cell under conditions such that said nucleic acid sequence of interest is expressed in said transgenic plant cell, and

d) identifying said transgenic plant cell.

The claims of this patent are directed to:

United States of America as represented by the Secretory of Agriculture & University of Hawaii

US 6686513

  • Earliest priority – 19 Mar 1998
  • Filed – 20 Oct 2000
  • Granted – 03 Feb 2004
  • Expected expiry – 17 Mar  2019
Title – Sugarcane ubi9 gene promoter sequence and methods of use thereof

Claim 1
A transgenic plant cell comprising a promoter comprising SEQ ID NO:10, wherein said promoter is operably linked to a nucleic acid sequence of interest, and said plant cell is selected from sugar cane, tobacco, sorghum, pineapple, rice, maize, tomato, soybean, banana, and garlic.
Claim 6
A method for expressing a nucleic acid sequence of interest in a plant cell selected from sugar cane, tobacco, sorghum, pineapple, rice, maize, tomato, soybean, banana, and garlic, comprising:a) providing:

i) said plant cell;

ii) a nucleic acid sequence of interest; and

iii) a promoter comprising SEQ ID NO: 10;

b) operably linking said nucleic acid sequence of interest to said promoter to produce a transgene; and

c) introducing said transgene into said plant cell to produce a transgenic plant cell under conditions such that said nucleic acid sequence of interest is expressed in said transgenic plant cell.

This patent is a Continuation in part of US 6706948.

US 6638766

  • Earliest priority – 19 Mar 1998
  • Filed – 24 May 2001
  • Granted – 28 Oct 2003
  • Expected expiry – 4 Jun  2019 (adjusted)
Title – Promoter of the sugarcane UBI4 gene

Claim 1
A substantially purified nucleic acid sequence comprising the nucleotide sequence selected from the group consisting of SEQ ID NO:7 and the complement thereof.
Claim 4
A transgenic plant cell comprising a nucleic acid sequence comprising the double-stranded nucleotide sequence listed as SEQ ID NO:7, wherein said nucleotide sequence is operably linked to a nucleic acid sequence of interest.

This patent is a Continuation of US 6706948.

Remarks

Related patent applications were also filed in Australia (AU 3192799 A1), Europe (EP 1063880 A1), Canada (CA 2324520) and Israel (IL 138580). However, the applications in Australia, Europe and Canada all lapsed.

Note: Patent information was last updated on 31 October 2006. Search terms: “polyubiquitin” in abstract. Patent database: PatentLens in combination with INPADOC.