Cold-inducible promoters
Promoters responsive to low or cold temperatures (equal to or below 10°C) are the subject matter of patents and patent applications filed by the Japanese companies Takara Shuzo Co., Ltd and Japan Tobacco Inc., The University of Quebec in Montreal and Danisco.
Most of the cited institutes claim in their patents a particular “cold” promoter and its variant sequences.
A. Takara Shuzo Co., Ltd’s patents and patent applications
The United States granted patents and the European and Canadian patent applications relate to a vector useful for the expression of a protein under low temperature conditions. The vector of the invention contains:
- a promoter regulatory region; and
- a 5′-untranslated region derived from a cold-shock protein gene.
The promoter region and 5′ untranslated region of the vector are not limited to a particular promoter. In addition a promoter and a 5′-untranslated region are derived from the cold-shock protein (csp) A of Escherichia coli. The nucleotide sequence of such promoter is also claimed by the inventors.
Patent Number | Title, Independent Claims and Summary of Claims | Assignee | |||
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US 6479260
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Title – Low-temperature inducible expression vector
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Takara Shuzo Co., Ltd (now Takara Holdings Inc.) |
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US 6897042
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Title – Low-temperature inducible expression vector
This patent is a Countinuation of US 6479260. |
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EP 1033408 A1
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Title – Cold-inducible expression vector
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CA 2309600 AA
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Title – Cold-inducible expression vector
The claims of this Canadian patent application are the same as EP 1033408 (above). |
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Remarks |
The related Australian application AU 10546/99 lapsed on August 3, 2000. |
Note: Patent information was last updated on 10 May 2006.
Search terms: “promoter” in abstract and “Takara Shuzo” in applicant.
Patent database: PatentLens.
B. Patents on particular cold-temperature promoters
As mentioned above, the patents filed by most institutes relate to a specific low temperature-responsive promoter.
The following table summarizes some bibliographic data of the patents and patent applications filed by the cited entities and a brief description of the promoter claimed.
Patent number | Title, Independent Claims and Summary of Claims | Assignee | |||||
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US 6084089
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Title – Cold-inducible promoter sequences
DNA sequences of a promoter isolated from potato. Partial and modified sequences of the promoter with cold-inducible promoter activity are also claimed. According to the inventors, “cold-inducible” promoter means induced at a temperature not higher than 6°C and expression of the gene controlled by such promoter maintained for not less than 5 months. |
Japan Tobacco |
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AU 725727 B2
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Title– Cold-inducible promoter sequences
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EP 812917 B1
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Title – Cold-inducible promoter sequences
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Remarks |
The related patents were also granted in Japan (JP 3469902), China (CN 96192162.5) and Canada (CA 2213991). | ||||||
US 6184443
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Title – Cold-inducible promoter and tuber-specific promoter sequence from potato alpha amylase gene
DNA sequences (variant, homologue and fragments) of an isolated alpha-amylase promoter from potato (Solanum tuberosum) having cold-sensitive activity. The promoter has tuber tissue specific activity. |
Danisco |
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Remarks |
Related patent applications in Europe (EP 787194 A1) and Canada (CA 2202896 AA) were withdrawn and Lapsed, respectively. Related Australian (AU 27881/95) application has also lapsed. | ||||||
US 5847102
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Title – Cold-induced promoter from winter Brassica napus
Isolated DNA sequences comprising a promoter and 5′ regulatory region of the low temperature responsive gene BN115 from Brassica napus. Sequences that hybridize to the promoter sequence and exhibit low temperature activity are also part of the claimed invention. An enhancer and a negative regulatory element present in the regulatory region are also claimed. |
Canadian Ministry of Agriculture |
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CA 2146712
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Title – Cold-induced promoter from winter Brassica napus
The claims are the same as US 5847102. |
Note: Patent information was last updated on 12 May 2006.
Search terms for Japan Tobacco patents: “promoter” in abstract and “Japan Tobacco” in applicant.
Search terms for Danisco patents: “promoter” in abstract and “Danisco” in applicant.
Search terms for Canadian Ministry of Agriculture patents: “promoter” in abstract and “Canadian Ministry” in applicant.
Patent database: PatentLens in combination with INPADOC.