Promoters based on glucocorticoid receptor (GR)
One of the best characterized steroid-responsive promoter systems is the GR. It has been used widely in transgenic animal cells, in particular mammalian cells. Granted patents claiming the basic principle of the glucocorticoid-responsive transcriptional factors were not found. Neither were patents or patent applications claiming a GR-based system for plants.
The system for expression in animal cells is part of an invention disclosed in a U.S. patent granted to McGill University.
The invention comprises an animal expression vector containing a synthetic promoter:
- having at least 5 glucocorticoid response elements (GREs)
- GREs are placed upstream of a viral or mammalian TATA box.
- The vector also contains a transcriptional initiator site 20 to 50 bases from the TATA box
- a restriction endonuclease site for the insertion of a gene to be expressed from the promoter and
- lacks elements which bind to nuclear factor 1.
The promoter itself, which is also claimed, is responsive to several steroid receptors including glucocorticoid, progesterone, androgen and mineralocorticoid receptor. There is no limit on the types of cells into which the promoter is transfected.
Specific Patent Information
Title, Independent Claims and Summary of Claims
||Title – Expression responsive to steroid hormones
|The related PCT application WO 94/28150 and the Australian application AU 67918/94 were withdrawn in 1996.|
Note: Patent information on this page was last updated on 21 April 2006.