Enhancers from the ocs gene – Specific Patent Information

Patent Number Title, Independent Claims and Summary of Claims Assignee
US 5573932

  • Earliest priority – 6 February 1987
  • Filed – 18 May 1990
  • Granted – 12 November 1996
  • Expected expiry – 12 November 2013
Title – OCS element

Claim 1
A recombinant DNA molecule comprising
a DNA fragment which fragment is a plant enhancer element capable of being bound by an OCS transcription factor and displaying the upper band binding pattern characteristic of the wild-type ocs enhancer in gel retardation assays, said fragment consisting essentially of a consensus sequence selected from the group consisting of 5′-T-G-A-C-G-T(C)-A-A-G-C(G)-G(A)-A(C)-T-G(T)-A-C-G-T(C)-A(C)-A(C)-3′ and its reverse sequence, in combination with
(a) a plant-expressible promoter heterologous to said plant enhancer element wherein said promoter is placed 3′ to said enhancer element, and
(b) a plant-expressible structural gene wherein said gene is placed 3′ to said promoter and under the regulatory control of said enhancer element and said plant-expressible promoter.
Claim 16
A recombinant DNA molecule comprising
a DNA fragment which fragment is a plant enhancer element capable of being bound by an OCS transcription factor and displaying the upper band binding pattern characteristic of the wild-type ocs enhancer in gel retardation assays, wherein said fragment comprises the sequence 5′-ACGTAAGCGCTTACGT-3′ and its reverse sequence, in combination with
(a) a plant-expressible promoter heterologous to said plant enhancer element wherein said promoter is placed 3′ to said enhancer element, and
(b) a plant-expressible structural gene wherein said gene is placed 3′ to said promoter and under the regulatory control of said enhancer element and said plant-expressible promoter.

The claims are generally drawn to:

  • A recombinant DNA molecule comprising in a 5′-3′ direction an OCS enhancer with an opine synthase enhancer consensus sequence, a plant-expressible promoter and a plant-expressible structural gene. The structural gene is under the control of the enhancer and the promoter.
  • A recombinant DNA molecule having the same elements as mentioned above, but the enhancer element has the sequence 5′-ACGTAAGCGCTTACGT-3

Mycogen Plant Sciences, Inc

(now owned by Dow AgroSciences)

US 5710267

  • Earliest priority – 6 February 1987
  • Filed – 2 June 1995
  • Granted – 20 January 1998
  • Expected expiry – 20 January 2015
Title – OCS element

Claim1
A recombinant DNA molecule comprisinga DNA fragment which fragment is a plant enhancer element capable of being bound by an OCS transcription factor and displaying the upper band binding pattern characteristic of the wild-type ocs enhancer in gel retardation assays, said fragment comprising a consensus sequence selected from the group consisting of 5′-A-C-G-T-A-A(C)-G-C-G-A(G)-T(A)-G(C)-A-C(T)-G-T(C)-3′ and its reverse sequence, in combination with 
(a) a plant-expressible promoter heterologous to said plant enhancer element wherein said promoter is placed 3′ to said enhancer element, and 
(b) a plant-expressible structural gene wherein said gene is placed 3′ to said promoter and under the regulatory control of said enhancer element and said plant-expressible promoter.
Claim 16
A recombinant DNA molecule comprisinga DNA fragment which fragment is a plant enhancer element capable of being bound by an OCS transcription factor and displaying the upper band binding pattern characteristic of the wild-type ocs enhancer in gel retardation assays, wherein said fragment comprises the sequence 5′-ACGTAAGCGCTTACGT-3′ and its reverse sequence, in combination with
(a) a plant-expressible promoter heterologous to said plant enhancer element wherein said promoter is placed 3′ to said enhancer element, and
(b) a plant-expressible structural gene wherein said gene is placed 3′ to said promoter and under the regulatory control of said enhancer element and said plant-expressible promoter.
Claim 18
A recombinant DNA molecule comprisinga DNA fragment which fragment is a plant enhancer element capable of being bound by an OCS transcription factor and displaying the upper band binding pattern characteristic of the wild-type ocs enhancer in gel retardation assays, said fragment comprising at leaset two consensus sequences selected from the group consisting of 5′-A-C-G-T-A-A(C)-G-C-G-A(G)-T(A)-G(C)-A-C(T)-G-T(C)-3′ and its reverse sequence, in combination with
(a) a plant-expressible promoter placed 3′ to said enhancer element, and 
(b) a plant-expressible structural gene wherein said gene is placed 3′ to said promoter and under the regulatory control of said enhancer element and said plant-expressible promoter.
Claim 33
A recombinant DNA molecule comprisinga DNA fragment which fragment is a plant enhancer element capable of being bound by an OCS transcription factor and displaying the upper band binding pattern characteristic of the wild-type enhancer in gel retardation assays, wherein said fragment comprises the sequence 5′-ACGTAAGCGCTACGT-3′ and its reverse sequence, in combination with
(a) a plant-expressible promoter heterologous to said plant enhancer element wherein said promoter is placed 3′ to said enhancer element, and 
(b) a plant-expressible structural gene wherein said gene is placed 3′ to said promoter and under the regulatory control of said enhancer element and said plant-expressible promoter.

This patent is a Continuation of US 5573932.

The claims are generally directed to three different recombinant DNA molecules having the same elements as mentioned in US 5573932. The difference is in the nucleotide sequence of the enhancer element.

Agrigenetics LP (owned byMycogen) & CSIRO

US 5837849

  • Earliest priority – 6 February 1987
  • Filed – 2 June 1995
  • Granted – 17 November 1998
  • Expected expiry – 17 November 2015
Title – OCS element

Claim 1
An isolated DNA fragment which is a plant enhancer capable of activating or enhancing the transcription level of a plant-expressible gene, said enhancer element comprising a consensus sequence selected from the group consisting of 5′-T-G-A-C-G-T(C)-A-A-G-C(G)-G(A)-A(C)-T-G(T)-A-C-G-T(C)-A(C)-A(C)-3′ and its reverse sequence.

This patent is a divisional of US 5573932.

The claim is drawn to an isolated plant enhancer element capable of enhancing the expression of a gene of interest in a plant having the consensus sequence 5′- TGACGTAAGCGATGACGTAA-3′

EP 278659 B1

  • Earliest priority – 6 February 1987
  • Filed – 2 February 1988
  • Granted – 24 May 1995
  • Expected expiry – 2 February 2008
Title – OCS enhancer

Claim 1
A monocotyledonous plant transformed with a recombinant DNA molecule comprising a plant transcription activating element capable of activating or enhancing the transcription level of a gene comprising a sequence having 50% to 100% homology with an identifying sequence selected from 5′-ACGTAAGCGCTTACGT-3′ and its reverse sequence.
Claim 15
A method for enhancing the expression of a plant-expressible gene in plant tissue comprising the steps of
(a) inserting a transcription activating element comprising a sequence having 50% to 100% homology to an identifying sequence selected from 5′-ACGTAAGCGCTTACGT-3′ and its reverse sequence, in such a way that said transcription activating element modulates the expression of said gene, and
(b) introducing said recombinant DNA molecule into plant tissue.

The claims are directed to:

  • A monocot transformed with a recombinant DNA molecule having an enhancer element with a sequence that is 50 to 100% homologous to the sequence 5′-ACGTAAGCGCTTACGT-3′.
  • A method for enhancing the expression of a gene in a monocot by introducing a recombinant molecule with an enhancer element as described above.

Originally assigned  to Lubrizol Enterprises, Inc.
and CSIRO

(Now owned by Agrigenetics
and CSIRO according to Online Public File Inspection)

Remarks

Patents have lapsed in Austria, Greece, Belgium, France, Gemany, Netherlands, Spain, Sweden and UK.

Applications were also filed in Japan (JP 63276492 A2) and South Africa (ZA 8800319 A).

CA 1309365

  • Earliest priority – 6 February 1987
  • Filed – 5 February 1988
  • Granted – 27 October 1992
  • Expected expiry – 27  October  2009

Title – OCS enhancer

Claim 1
A recombinant DNA molecule comprising a plant transcription activating element capable of activating or enhancing the transcription level of a gene comprising a sequence having about 50% to 100% homology with an identifying sequence selected from 5′-ACGTAAGCGCTTACGT-3′ and its reverse sequence.
Claim 32
A method for enhancing the expression of a plant-expressible gene in plant tissue comprising the steps of
(a) inserting a transcription activating element comprising a sequence having about 50% to 100% homology to an identifying sequence selected from the group consisting of 5′-ACGTAAGCGCTTACGT-3′ and its reverse sequence into a recombinant DNA molecule comprising a plant-expressible gene under the control of a promoter in such a way that said transcription activating element modulates the expression of said gene, and 
(b) introducing said recombinant DNA molecule into plant tissue.

The claims are the same as the claims of the European patent EP 278659 B1 except that transformation of a plant by the recombinant molecule is not limited to monocots.

Lubrizol Enterprises, Inc. (It is most likely part of Mycogen Seeds now)
and CSIRO

Note: Patent information was last updated on 22 May 2006. Search terms: “ocs element”  in abstract. Patent database: PatentLens in combination with INPADOC.