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Patents on duplicated CaMV 35S enhancer sequences
Patent number Title, Independent Claims and Summary of Claims Assignee
US 5164316

  • Earliest priority – 13 January 1987
  • Filed – 17 August 1989
  • Granted – 17 November 1992
  • Expected expiry – 17 November 2009
 Title – DNA construct for enhancing the efficiency of transcription

Claim 1A plant cell comprising: a DNA construct having as components, (a) a duplicated CaMV 35s enhancer sequence comprising an AluI-EcoRV fragment of a CaMV 35S upstream region; and (ii) a promoter comprising an RNA polymerase binding site and an mRNA initiation site; (b) a nucleotide sequence of interest for transcription to mRNA; and (c) a termination region wherein said components are operably joined.

Listed in the patent documents as the University of British Columbia, but assigned to Monsanto in 1993
US 5196525

  • Earliest priority – 13 January 1987
  • Filed – 8 April 1991
  • Granted – 23 March 1993
  • Expected expiry -23 March 2010
 Title – DNA construct for enhancing the efficiency of transcription

Claim 1
A DNA construct having as components, (a) a transcription initiation region including (i) a tandemly duplicated CaMV 35S enhancer sequence comprising an AluI-EcoRV fragment of a CaMV 35S upstream region; (ii) a promoter comprising an RNA polymerase binding site and an mRNA initiation site; (b) a nucleotide sequence of interest for transcription to mRNA; and (c) a termination region wherein said components are operably joined.
US 5322938

  • Earliest priority – 13 January 1987
  • Filed – 17 November 1992
  • Granted – 21 June 1994
  • Expected expiry – 21 June 2011
 Title – DNA sequence for enhancing the efficiency of transcription

Claim 1
A chimeric transcriptional initiation region comprising: as operably joined components (i) a tandemly duplicated CaMV 35S enhancer sequence comprising an AluI-EcoRV fragment of a CaMV 35S upstream region; and (ii) a promoter comprising an RNA polymerase binding site and an mRNA initiation site, wherein when a nucleotide sequence of interest is transcribed under the regulatory control of said chimeric transcriptional initiation region, the amount of transcription product is enhanced as compared to the amount of transcription obtained with a chimeric transcriptional initiation region comprising a single copy of said CaMV enhancer sequence and said promoter.

Monsanto
US 5359142

  • Earliest priority – 13 January 1987
  • Filed – 9 March 1994
  • Granted –  25 October 1994
  • Expected expiry – 25 October 2011
 Title – Method for enhanced expression of a protein

Claim 1
A differentiated plant comprising that comprise a DNA construct having as components (a) a tandemly duplicated CaMV 35S enhancer sequence comprising an AluI-EcoRV fragment of a CaMV 35S upstream region; and (b) a promoter comprising an RNA polymerase binding site and an mRNA initiation site; (c) a nucleotide sequence of interest; and (d) a termination region; wherein said components are operably joined.

This patent is a divisional of US 5322938.

  • A differentiated plant comprising the DNA construct as generally claimed in US 5196525.
US 5424200

  • Earliest priority – 13 January 1987
  • Filed – 11 July 1994
  • Granted – 13 June 1995
  • Expected expiry – 13 June 2012
 Title – Method for enhanced expression of a DNA sequence of interest

Claim 1
A method for expressing a DNA sequence of interest comprising transforming a plant cell with a DNA construct comprising (a) a tandemly duplicated CaMV 35S enhancer sequence comprising an AluI-EcoRV fragment of a CaMV 35S upstream region; (b) a promoter comprising an RNA polymerase binding site and an mRNA initiation site; (c) a nucleotide sequence of interest; and (d) a termination region; wherein said components are operably joined so that said sequence of interest is transcribed.

This patent is a divisional of US 5359142.

  • A method for expressing a DNA sequence of interest by transforming a plant cell with a DNA construct as claimed in US 5196525.
CA 1325191

  • Earliest priority — 13 January 1987
  • Filed – 12  January 1988
  • Granted – 14 December 1993
  • Expected expiry – 14 December 2010
 Title – DNA construct for enhancing the efficiency of transcription

Claim 1
A transcriptional initiation region comprising:
(a) a plant or viral enhancer domain comprising a plurality of units each including a sequence of the group comprising: GTGG, ATGG, GTAG, GTGA, GTGGA(T)A(T)A(T),GTGTGGA(T)A(T)A(T)G, and complementary sequence thereof, said enhancer domain further comprising at least one additional unit including a sequence selected from the said group; and
(b) a transcription initiation domain comprising an RNA polymerase binding site and an mRNA initiation site, under the enhancing control of said enhancer domain.
Claim 6
A DNA construct comprising: a transcription initiation region comprising:
(a) an enhancer domain comprising a plurality of units each including a sequence selected from the group comprising: GTGG, ATGG, GTAG, GTGA, GTGGA(T)A(T)A(T),GTGTGGA(T)A(T)A(T)G, and complementary sequence thereof;  and
(b) a transcription initiation domain comprising an RNA polymerase binding site and an mRNA initiation site under the enhancing control of said enhancer domain;
(c) a sequence of interest for transcription to mRNA; and
(d) a termination region.
Claim 22
A DNA sequence comprising a transcription initiation region capable of regulating transcription of a nucleotide sequence of interest in a plant cell and including, as operably joined components, (i) two to four copies in tandem of an enhancer sequence comprising an AluI-EcoRV fragment of a CaMV 35S upstream region; and (ii) a promoter comprising an RNA polymerase binding site and an mRNA initiation site.
Claim 29
A chimeric transcriptional initiation region comprising: as operably joined components, (i) two or more copies of a CaMV 35S enhancer sequence and (ii) a promoter comprising an RNA polymerase binding site and an mRNA initiation site, wherein when a nucleotide sequence of interest is transcribed under the regulatory control of said chimeric transcriptional initiation region, the amount of transcription product is enhanced as compared to the amount of transcription obtained with a chimeric transcriptional initiation region comprising a single copy of said CaMV enhancer sequence and a promoter.
Claim 33
A DNA construct having as components, (a) a transcription initiation region including (i) a tandemly duplicated CaMV 35S enhancer sequence comprising an AluI-EcoRV fragment of a CaMV 35S upstream region; (ii) a promoter comprising an RNA polymerase binding site and an mRNA initiation site; (b) a nucleotide sequence of interest for transcription to mRNA; and (c) a termination region wherein said components are operably joined.
Claim 38
A method for producing a plant cell capable of enhanced expression of a nucleotide sequence of interest, said method comprising: operably linking to form an expression cassette said nucleotide sequence of interest and a transcription initiation region capable of regulating transcription of a DNA sequence of interest in a plant cell, wherein said transcription initiation region comprises (i) two to four copies in tandem of an enhancer sequence comprising an AluI-EcoRV fragment of a CaMV 35S upstream region; and (ii) a promoter comprising an RNA polymerase binding site and an mRNA initiation site; and introducing said expresssion cassette into said plant cell to form a plant cell capable of enhanced expression of a said nucleotide sequence of interest.
Claim 47
A plant cell comprising: a DNA construct having components, (a) a transcription initiation region including (i) a tandemly duplicated CaMV 35S enhancer sequence comprising an AluI-EcoRV fragment of a CaMV 35S upstream region; (ii) a promoter comprising an RNA polymerase binding site and an mRNA initiation site; (b) a nucleotide sequence of interest for transcription to mRNA; and (c) a termination region wherein said components are operably joined.

Claim elements:

  • A DNA transcription region comprising 2 to 4 copies in tandem of enhancer 35S and a promoter region as generally claimed in US 5164316.
  • A chimeric transcriptional initiation region comprising 2 or more copies of enhancer 35S and having the properties as generally claimed in US 5322938.
  • A DNA construct as generally claimed in US 5196525.
  • A method of producing a plant cell capable of enhanced expression of a gene of interest by having in an expression cassette with a gene of interest linked to a DNA transcription region as described in the first point.
  • A plant cell comprising a DNA construct as generally claimed in US 5164316.

Remarks

 A related patent has been granted in Brazil (BR 1101045).

Note: Patent information on this page was last updated on 6 March 2006.