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Peas

Summary

FreshWorld has a granted patent in the United States related to a method for transforming plumule material or cotyledons of a pea seed, either from a garden pea plant or an edible pod, with A. tumefaciens or A. rhizogenes carrying a gene of interest. A related Australian application was abandoned.

Specific Patent Information

Patent Number Title, Independent Claims and Summary of Claims Assignee
US 5286635

  • Earliest priority – 24 September 1991
  • Filed – 24 September 1991
  • Granted – 15 February 1994
  • Expired – 15 March 2006
 Title – Genetically transformed pea plants and methods for their production

Claim 1A method for genetically transforming a pea plant, said method comprising:

A) culturing explant material from the seed plumule of the pea plant with Agrobacterium tumefaciens or rhizogenes cells carrying an exogenous DNA sequence, wherein the strain of Agrobacterium is selected to be capable of infecting and transferring DNA to the explant material;
B) regenerating shoots from the explant material from step (A), wherein said shoots are obtained from non-callus material;
C) selecting regenerated shoots from step (B) which express the exogenous DNA sequence, and
D) rooting said regenerated shoots to produce viable pea plants expressing the exogenous DNA sequence.
Claim 7A method for genetically transforming a pea plant, said method comprising:

A) obtaining plumule material from a pea plant seed;
B) culturing the plumule material with Agrobacterium tumefaciens or rhizogenes cells carrying an exogenous DNA sequence in a co-cultivation medium for a time and under conditions selected to effect transfer of the exogenous DNA sequence to cells of the plumule material, wherein the strain of Agrobacterium is selected to be capable of infecting and transferring DNA to the explant material;
C) separating the Agrobacterium cells form the plumule material;
D) regenerating shoots from the plumule material from step (C) in a regeneration medium for a time and under conditions selected to produce shoots from non-callus regions of the plumule;
(E) selecting regenerated shoots from step (D) which express the exogenous DNA sequence; and
(F) rooting the selected regenerated shoots from step (E) in a rooting medium to produce plants which express the exogenous DNA sequence.
Claim 19A method for genetically transforming a pea plant, said method comprising:

A) preparing an explant by removing the seed coat from a sterilized seed of the pea plant and cutting off the cotyledons where they are attached to the embryo;
B) culturing explant material from sterilized seed of the pea plant with Agrobacterium tumefaciens or rhizogenes cells carrying an exogenous DNA sequence, wherein the strain of Agrobacterium is selected to be capable of infecting and transferring DNA to the explant material;
C) regenerating shoots from the explant material from step (A), wherein said shoots are obtained from non-callus material;
D) selecting regenerating shoots from step (B) which express the exogenous DNA sequence, and
E) rooting said regenerated shoots to produce viable pea plants expressing the exogenous DNA sequence.

Granted US 5286635 has expired due to non-payment of maintenance fees according to the USPTO.

Method for transforming the plumule of a pea seed and cotyledons with A. tumefaciens or A. rhizogenes having an exogenous gene. Shoot regeneration and production of viable pea plants expressing the gene of interest are also claimed.

 FreshWorld
WO 1993/06209 A1

  • Earliest priority – 24 September 1991
  • Filed – 9 September 1992
  • OPI – 1 April 1993
 Title – Genetically transformed pea plants and methods for their production

Claim 1

A method for genetically transforming a pea plant, said method comprising:
(a)  culturing explant material from the pea plant with Agrobacterium cells carrying an exogenous DNA sequence;
(b) regenerating shoots from the explant material from step (a), wherein said shoots are obtained from non-callus material; and
(c) selecting regenerated shoots from step (b) which express the exogenous DNA sequence.
Claim 8

A method for genetically transforming a pea plant, said method comprising:
(a) culturing explant material from the seed plumule of the pea plant with Agrobacterium cells carrying an exogenous DNA sequence;
(b) regenerating shoots from the explant material from step (a); and
(c) selecting regenerated shoots from step (b) which express the exogenous DNA sequence.
Claim 15
A method for genetically transforming a pea plant, said method comprising:(a) obtaining a plumule material from pea plant seed;
(b) culturing the plumule material with Agrobacterium cells carrying an exogenous DNA sequence in a cocultivation medium for a time and under conditions selected to effect transfer of the exogenous DNA sequence to cells of the plumule material;
(c) separating the Agrobacterium cells from the plumule material;
(d) regenerating shoots from the plumule material from step (c) in a regeneration medium for a time and under conditions selected to produce shoots from non-callus regions of the plumule;
(e) selecting regenerated shoots from step (d) which express the exogenous DNA sequence; and
(f) rooting the selected regenerated shoots from step (e) in a rooting medium.
Claim 33

A pea plant which expresses a heterologous gene.
Remarks National phase entry of WO 1993/06209 in Australia (AU 26441/92) has lapsed on 16 June 1994.

Note: Patent information on this page was last updated on 21 March 2006.