Menu

About Us

Cambia is an independent non-profit institute creating new technologies, tools and paradigms to promote change and enable innovation.

Read More

Strawberry

Summary

Update July 2003

A United States patent was issued to (VPP Corp) DNA Plant Technology Corp. with granted claims disclosing a method to produce transgenic strawberry plants by co-cultivating Agrobacterium carrying a gene of interest with explants in the presence of glucose or fructose.

The US patent is broad with respect to the target tissue used in transformation. The main limitation of the patent is with additives to the culture medium. If the procedure is carried out in medium containing a carbohydrate that is not glucose or fructose or no carbohydrate, it is unlikely there would be infringement.

Specific Patent Information

Patent Number Title, Independent Claims and Summary of Claims Assignee
US 6274791

  • Earliest priority – 19 January 1998
  • Filed – 15 January 1999
  • Granted – 14 August 2001
  • Expected expiry – 15 January 2019
 Title – Methods for strawberry transformation using Agrobacterium tumefaciens

Claim 1
A method of preparing transgenic strawberry shoots, the method comprising:

(a) contacting strawberry explants with Agrobacterium tumefaciens in a co-cultivation medium prepared using glucose or fructose, thereby producing transformed strawberry explants; and
(b) culturing the transformed strawberry explants in a selection medium prepared using glucose or fructose, thereby producing transformed strawberry shoots.

Granted United States patent US 6274791 recites an Agrobacterium tumefaciens-mediated transformation method of strawberry, where the co-cultivation and selection medium includes glucose or fructose.

DNA Plant Technology Corp.
WO 1999/35903 A1

  • Earliest priority – 19 January 1998
  • Filed – 15 January 1999
  • OPI – 22 July 1999
 Title – Methods for strawberry transformation using Agrobacterium tumefaciens

Claim 1
A method for the preparation of transgenic strawberry explants comprising contacting strawberry explants with Agrobacterium tumefaciens in a co-cultivation medium containing glucose or fructose.
Claim 27
A method for the preparation of transgenic strawberry shoots comprising culturing transformed strawberry explants in selection medium containing glucose or fructose.
Claim 35
A method for the preparation of transgenic strawberry plants comprising culturing transformed strawberry shoots in rooting medium containing glucose or fructose.

PCT application WO 1999/35903 recites use of glucose or fructose in the medium that is used for transforming strawberry with Agrobacterium.
Remarks National phase entry of WO 1999/35903 in Australia (AU 23231/99) has lapsed on 5 October 2000.

Note: Patent information on this page was last updated on 27 March 2006.