Barley – Patent assigned to Sapporo Breweries Ltd (JP)
Barley is one of the major cereal crops worldwide and as such biotechnological genetic improvement technologies are a desirable avenue for the introduction of novel traits, like disease resistance or modified starch production.
This patent discloses a method for transformation of barley using Agrobacterium in combination with acetosyringone (a phenolic compound that induces DNA transfer by Agrobacterium) and suspending this mixture together with callus cells as target tissue for transformation.
Specific Patent Information
|Patent Number||Title, Independent Claims and Summary of Claims||Assignee|
|US 6291244 B1
||Title – Method of producing transformed cells of barley
The present invention provides variations on existing protocols for the transformation of cereals and other monocots. In the case of barley the concentration of the widely used phenolic compound acetosyringone has been adjusted to the special requirements given for the interaction between Agrobacterium and the plant cells to achieve optimal transformation efficiency. If sub-optimal concentrations are used or a different or no phenolic compound is utilized, thereby possibly sacrificing transformation efficiency, infringement can likely be avoided.
Sapporo Breweries Ltd (JP)
|Remarks||related patents and applications in Japan JP 2002509440 T2 (deemed withdrawn), Australia AU 79377/98 (lapsed) and WO 99/04618.|
Note: Patent information on this page was last updated on 6 February 2006.