Patent granted to Japan Tobacco Inc.
The United States patent granted to Japan Tobacco discloses a method commonly called co-transformation. In this method, two T-DNAs containing genes that encode different products (e.g. gene of interest and selectable marker) are inserted into the plant genome via Agrobacterium. The method results in transformed plants having the gene of interest and lacking the selectable marker gene.
Specific Patent Information
|Patent Number||Title, Independent Claims and Summary of Claims||Assignee|
||Title – Method for introducing two T-DNAs into plants and vectors therefor
The patent US 5731179 claims
The independent claims don’t limit the size of the DNA fragment contained by the second T-DNA.
The claims to the hybrid vector state that the T-DNAs are sufficiently far apart to allow them to be independently inherited. The patent disclosure doesn’t describe what minimum distance would allow this but does provide the example, where the borders are separated by an origin of replication and sequences that contain the virB and virG gene (i.e., about 15 kb) and the T-DNAs are independently inherited. The claims are a bit ambiguous because they don’t require independent segregation to occur at any particular frequency (e.g., 100% of the time? 50%? rarely?).
Some of the hybrid vector claims require specifically named plasmids disclosed in the specification. Deposit information is not provided in the disclosure of the US patent.
Japan Tobacco Inc.
|AU 733623 B2
||Title – Method for transforming plants and vector therefor
This granted patent is a divisional of now abandoned AU 11213/95 A1 (OPI of the national phase entry of WO 1995/16031).
|AU 771116 B2
||Title – Agrobacterium mediated method of plant transformation
This granted patent is a divisional of now granted AU 733623 B2.
Note: Patent information on this page was last updated on 2 March 2006.