Chrysanthemums

Summary

A transformed chrysanthemum plant capable of expressing a gene of interest is the subject of the invention granted to Florigene Europein the United States. In this patent, the explants or plant material transformed with Agrobacterium are limited to stem, leaf, peduncle, petiole, meristem and shoot apex. The invention discloses a method to regenerate a chrysanthemum shoot and a chrysanthemum plant expressing an exogenous gene.

Specific Patent Information

Patent Number Title, Independent Claims and Summary of Claims Assignee
US 5567599

  • Earliest priority – 21 August 1990
  • Filed – 26 January 1994
  • Granted – 22 October 1996
  • Expected expiry – 25 January 2014
Title – Method for producing transformed chrysanthemum plants

Claim 1A method for producing a chrysanthemum plant comprising an exogenous DNA fragment, which chrysanthemum plant is capable of expressing a gene within the exogenous DNA fragment, comprising the steps of: A) isolating an explant from source material from a chrysanthemum plant, wherein said source material is selected from the group consisting of leaf, stem, peduncle, petiole, meristem, and shoot apex;
B) inoculating the explant from source material from the chrysanthemum plant with a culture of Agrobacterium, which Agrobacterium comprises an exogenous DNA fragment comprising a gene under the control of a promoter capable of promoting the transcription of the gene within the exogenous fragment, to obtain an inoculated explant;
C) incubating the inoculated explant of step (B) on an incubation medium;
D) culturing the incubated explant of step (C) on a regeneration medium to obtain regenerated shoots of the chrysanthemum plant; and
E) culturing the regenerated shoots of step (D) on a rooting medium to obtain a chrysanthemum plant comprising the exogenous DNA fragment, in which the chrysanthemum plant is capable of expressing the gene within the exogenous DNA fragment.
Claim 60
A method for producing a chrysanthemum plant comprising an exogenous DNA fragment using Agrobacterium as a vector, comprising the steps of:A) incubating chrysanthemum plant source material on a pretreatment medium;
B) isolating a leaf explant from source material from the chrysanthemum plant;
C) inoculating the explant from source material from the chrysanthemum plant with a culture of Agrobacterium, which Agrobacterium comprises an exogenous DNA fragment comprising a gene under the control of a promoter capable of promoting the transcription of the gene within the exogenous fragment, to obtain an inoculated explant;
D) incubating the inoculated explant of step (C) on an incubation medium;
E) culturing the incubated mixture of step (D) on a regeneration medium to obtain regenerated shoots of the chrysanthemum plant; and
F) culturing the regenerated shoots of step (E) on a rooting medium to obtain a chrysanthemum plant comprising the exogenous DNA fragment.

Production of a transformed chrysanthemum plant expressing a gene of interest by transforming different explants with Agrobacterium carrying the exogenous gene. The group of explants transformed include leaf, stem, peduncle, petiole, meristem and shoot apex. To obtain a chrysanthemum plant, shoot formation and rooting are induced.

Florigene Europe B.V.
Remarks National phase entry of WO 1992/03041 in Australia (AU 84330/91) has lapsed on 13 May 1993.

Note: Patent information on this page was last updated on 14 March 2006.

Chrysanthemum – Patent granted to Florigene Europe B.V.

Actual granted claims

US 5 567 599
Claim 1A method for producing a chrysanthemum plant comprising an exogenous DNA fragment, which chrysanthemum plant is capable of expressing a gene within the exogenous DNA fragment, comprising the steps of: A) isolating an explant from source material from a chrysanthemum plant, wherein said source material is selected from the group consisting of leaf, stem, peduncle, petiole, meristem, and shoot apex;
B) inoculating the explant from source material from the chrysanthemum plant with a culture of Agrobacterium, which Agrobacterium comprises an exogenous DNA fragment comprising a gene under the control of a promoter capable of promoting the transcription of the gene within the exogenous fragment, to obtain an inoculated explant;
C) incubating the inoculated explant of step (B) on an incubation medium;
D) culturing the incubated explant of step (C) on a regeneration medium to obtain regenerated shoots of the chrysanthemum plant; and
E) culturing the regenerated shoots of step (D) on a rooting medium to obtain a chrysanthemum plant comprising the exogenous DNA fragment, in which the chrysanthemum plant is capable of expressing the gene within the exogenous DNA fragment.
Claim 60
A method for producing a chrysanthemum plant comprising an exogenous DNA fragment using Agrobacterium as a vector, comprising the steps of:A) incubating chrysanthemum plant source material on a pretreatment medium;
B) isolating a leaf explant from source material from the chrysanthemum plant;
C) inoculating the explant from source material from the chrysanthemum plant with a culture of Agrobacterium, which Agrobacterium comprises an exogenous DNA fragment comprising a gene under the control of a promoter capable of promoting the transcription of the gene within the exogenous fragment, to obtain an inoculated explant;
D) incubating the inoculated explant of step (C) on an incubation medium;
E) culturing the incubated mixture of step (D) on a regeneration medium to obtain regenerated shoots of the chrysanthemum plant; and
F) culturing the regenerated shoots of step (E) on a rooting medium to obtain a chrysanthemum plant comprising the exogenous DNA fragment.