Inhibition of Agrobacterium growth
PCT and US application filed by Monsanto
Overgrowth of Agrobacterium jeopardizes the survival of the transformed plant cells and also has an effect on the T-DNA transfer process. Insertion of multiple copies of a gene of interest into a plant cell is influenced by the frequency of T-DNA transfer into the cell. Agrobacterium-mediated transformation protocols strive to attain transformation events with a limited number of copies of DNA entering into any one cell. The presence of multiple inserts can lead to gene silencing or reduce expression levels of transformed genes, which is caused by several mechanisms including recombination between the multiple copies. Inhibiting agents of Agrobacterium growth should be effective against the bacterium but remain neutral with respect to plant cell growth.
Monsanto’s applications relate to the control of Agrobacterium growth during the transformation process in order to improve transformation efficiency. The use of inhibiting agents during inoculation and co-culture of Agrobacterium with a transformable plant cell results, according to the disclosure, in increased transformation efficiencies and a low copy number of an introduced genetic component in several plant systems. Preferred growth-inhibiting agents are compounds containing heavy metals such as silver nitrate or silver thiosulfate, antibiotics such as carbenicillin, and a combination of antibiotics and a clavulanic acid such as augmentin or timentin.
PCT Application Information
|Patent Number||Title, Independent Claims and Summary of Claims||Assignee|
||Title – A novel Agrobacterium-mediated plant transformation method
The claims as filed of the PCT application recite:
|US 2003204875 AA
||Title – Novel Agrobacterium-mediated plant transformation method
This application is a division of US 6603061, which is the priority document of WO 2001/09302. Claims of granted US 6603061 recite a method of Agrobacterium-mediated transformation that is limited to that transforming a corn plant (claim 1) or a dicotyledonous plant (claim 4).
Note: Patent information on this page was last updated on 6 March 2006.