Inhibition of enzymatic browning of Agrobacterium-transformed tissue

Patent applications filed by the University of Minnesota

Actual pending claims

US 20010034888 A1& WO 0144459 A2
Claim 1A method for transforming plant explant tissue, comprising:

A) contacting a cotyledon explant from a plant seedling infected with an Agrobacterium containing DNA to be introduced into the explant with an agent that inhibits enzymatic browning of a wounded plant, plant tissue or plant cell so as to yield transformed explant tissue; and
B) identifying transformed explant tissue.

Claim 22A method to identify an agent that enhances Agrobacterium -mediated transformation of a plant cell, plant tissue or plant part, comprising:

A) contacting the plant cell, plant tissue or plant part with Agrobacterium containing DNA to be introduced into the plant cell, plant tissue or plant part and the agent so as to yield a transformed plant cell, plant tissue or plant, wherein the agent is not a phenolic compound; and
B) detecting or determining whether the agent enhances Agrobacterium-mediated transformation of the plant cell, plant tissue or plant part relative to Agrobacterium-mediated transformation of a plant cell, plant tissue or plant part in the absence of the agent.

Claim 37A method for the stable transformation of plant tissue or cells, comprising:

A) contacting plant tissue or cells with an Agrobacterium containing DNA and an agent selected from the group consisting of a sulthydryl-containing agent, an iron chelator, a copper chelator, 10 an inhibitor of plant polyphenol oxidase and an inhibitor of plant peroxidase; and
B) identifying stably transformed plant tissue or cells.

Claim 44A plant medium comprising:
an amount of an agent effective to inhibit the enzymatic browning of a plant organ, tissue or cell, wherein the agent is selected from the group consisting of a sulfhydryl-containing agent, an iron chelator, a copper chelator, an inhibitor of polyphenol oxidase and an inhibitor of peroxidase.