Patent assigned to Rhône-Poulenc Agro

The invention disclosed in the following patent is directed to the use of an inflorescence of a monocot as a target tissue to be transformed with Agrobacterium. In the method, a dissected inflorescence or a callus derived from the dissected inflorescence is co-cultivated with Agrobacterium.

Specific Patent Information

Patent Number Title, Independent Claims and Summary of Claims Assignee
US 6037522

  • Earliest priority – 23 June 1998
  • Filed – 23 June 1998
  • Granted – 14 March 2000
  • Expected expiry – 22 June 2018
Title– Agrobacterium-mediated transformation of monocots

Claim 1A method of transforming a monocot comprising co-cultivating a monocot inflorescence with Agrobacterium containing a plasmid comprising a heterologous nucleic acid.
Claim 5A method of transforming a monocot comprising:

A) dissecting an inflorescence from a monocot;
B) initiating a callus from the inflorescence to generate an inflorescence-derived callus; and
C) co-cultivating the inflorescence-derived callus with Agrobacterium containing a plasmid comprising a heterologous nucleic acid.

Claim 13A method of making a transgenic monocot comprising:

A) dissecting an inflorescence from a monocot;
B) initiating a callus from the inflorescence to generate an inflorescence-derived callus;
C) co-cultivating the inflorescence-derived callus with Agrobacterium containing a plasmid comprising a heterologous nucleic acid; and
D) regenerating a transgenic monocot from the callus.

Claim 16A method of making a transgenic monocot comprising:

A) dissecting an inflorescence from a monocot;
B) co-cultivating the inflorescence with Agrobacterium containing a plasmid comprising a heterologous nucleic acid;
C) initiating a callus from the inflorescence; and
D) regenerating a transgenic monocot from the callus.

The United States patent US 6037522 claims

  • a method of transforming an inflorescence of a monocot  with Agrobacterium having heterologous DNA where:
  1.  the inflorescence to be transformed is first dissected and  then co-cultivated with Agrobacterium; or
  2.  the inflorescence to be transformed is first cultured in a medium to induce callus formation and then the callus is transformed withAgrobacterium.

Dependent claims also recite transgenic monocots, seeds or progeny of transgenic monocots produced by the methods.

The patent describes that “heterologous nucleic acid”  is nucleic acid which is not normally found in Agrobacterium T-DNA or the monocot that is to be transformed and includes all synthetically engineered and biologically derived genes which may be introduced into a plant by genetic engineering, including, but not limited, to nonplant genes, modified genes, synthetic genes, portion of genes, and genes from monocots and other plant species.

Rhône-Poulenc Agro

(now Aventis CropScience,
now owned by Bayer)

Remarks The related Australian application AU 46163/99 A1 was abandoned on March 15, 2001.

Note: Patent information on this page was last updated on 2 February 2006.