Maize
Summary
The University of Toledo (US), Pioneer Hi-Bred (US), and Stine Biotechnology (US) have been granted United States patents directed toAgrobacterium-mediated transformation of maize. The main difference among them is the type of maize tissue used as target for transformation:
- The University of Toledo claims a maize seedling wounded in a specific area for inoculation of the bacterium.
- Pioneer Hi Bred claims an immature embryo of maize and a medium formula for cultivation of a transformed maize embryo
- Stine Biotechnology also employs immature embryos as target tissue for transformation with Agrobacterium in their patent. Claims in their applications disclose a method to improve the production of so-called Type II callus (a preferred target tissue for the transformation of corn) by including a monosaccharide like glucose in addition to the usual sucrose in the medium.
Maize – Specific Patent Information – part 1
Patent Number | Title, Independent Claims and Summary of Claims | Assignee | ||||||
---|---|---|---|---|---|---|---|---|
US 5177010
|
Title – Process for Transforming Corn and the Products Thereof
Transformation of a corn seedling by inoculating vir+ A. tumefaciens in a wounded area located between the scutellar node to slightly beyond the coleoptile node. This area presents a high rate of cell division. |
University of Toledo |
||||||
US 2002/0002711 A1
|
Title – Process for Transforming Germinae and the Products Thereof
Transformed corn plant having a transformed gene produced by: Agrobacterium -mediated transformation or an equivalent method, direct cell transfer, regenerated protoplasts and single cell cultures. Fertile transgenic Zea mays having an exogenous gene and transformed Gramineae are also part of the filed claims. This patent application has been abandoned (failure to respond to a USPTO action) according to the USPTO. |
Goldman and Graves |
||||||
Remarks | A related patent has been granted in Japan (JP3234534, which is a divisional of now granted JP 2693443 claiming Agrobacterium-mediated transformation of Gramineae). | |||||||
US 5981840
|
Title – Method for Agrobacterium-Mediated Transformation
Transformation of an immature embryo of maize by contact and co-cultivation with Agrobacterium having a gene of interest. The media used for culturing the embryo contains N6 or MS salts, an antibiotic against Agrobacterium, and a selective agent for embryos expressing the gene. Regeneration of plants expressing the gene is also recited in the claims. |
Pioneer Hi-Bred |
||||||
AU 727849 B2
|
Title – Methods for Agrobacterium-mediated transformation
Claim 1 states the same method of maize transformation as that of US 5981840. |
|||||||
EP 971578 A1
|
Title– Methods for Agrobacterium-mediated Transformation
The transformation method disclosed in these applications is very similar to the method claimed in the United States patent US 5981840. The difference consists in the use of a co-cultivation medium free of LS salts. |
|||||||
Remarks |
|
Note: Patent information on this page was last updated on 7 February 2006.
Maize – Specific Patent Information – part 2
Patent Number | Title, Independent Claims and Summary of Claims | Assignee | |||
---|---|---|---|---|---|
US 6420630 B1
|
Title – Methods for tissue culturing and transforming elite inbreds of Zea mays L.
A method of transforming maize by co-cultivating Agrobacterium carrying a gene of interest with immature embryos. Heat shock is applied to enhance DNA integration. |
Stine Biotechnology |
|||
US 6919494 B2
|
Title – Methods for tissue culturing and transforming elite inbreds of Zea mays L.
Granted patent of application US 2002/0104131 (see below). Continuation of US Patent Application 09/203,679 (now US Patent 6420630 B1) |
||||
US 2002/0104131 A1
|
Title – Methods for tissue culturing and transforming elite inbreds of Zea mays L.
A method in which formation of Type II callus (a preferred form of target tissue for the transformation with Agrobacterium) from immature embryos is enhanced by adding sucrose and a monosaccharide to the medium. Continuation of US Patent Application 09/203,679 (now US Patent US 6420630 B1) |
||||
US 2002/0104132 A1
|
Title – Methods for tissue culturing and transforming elite inbreds of Zea mays L.
A method as in the application above but specifically designed for elite corn inbred lines. Divisional application from US Patent Application 09/203,679 (now US Patent US 6420630 B1), this application has been abandoned due to failure to respond to a USPTO action according to the USPTO. |
||||
US 2005/0278802 A1
|
Title – Methods for tissue culturing and transforming elite inbreds of Zea mays L.
This is a divisional application of now granted US 6420630. All of the independent claims preceeding claim 35 has been cancelled. |
Note: Patent information on this page was last updated on 7 February 2006.