Patents and application assigned to Japan Tobacco-Patents and application assigned to Japan Tobacco

This family of patents and one application disclose the use of an immature embryo of a monocot for Agrobacterium-mediated transformation. Within the embryo, the tissue to be transformed is the scutellum, which is the name given to the cotyledon of monocot plants. The transformed scutellum can be induced to become dedifferentiated calli that have the ability to regenerate normal plants after transformation.

In addition, they disclose an Agrobacterium used for transformation that contains a Ti or Ri (root-inducing) plasmid with a desired gene and a plasmid having a virulence region derived from the A. tumefaciens Ti plasmid pTiBo542.

Specific Patent Information

Patent Number Title, Independent Claims and Summary of Claims Assignee
AU 687863 B

  • Earliest priority – 3 September 1993
  • Filed – 1 Sept 1994
  • Granted – 5 March 1998
  • Expected expiry – 31 Aug 2014
Title – Method of transforming monocotyledon by using scutellum of immature embryo

Claim 1A method for transforming monocotyledons comprising transforming scutellum of an immature embryo of a monocotyledon with a bacterium belonging to genus Agrobacterium containing a desired gene, which immature embryo has not been subjected to a dedifferentiation treatment, to obtain a transformant.

The claims of the Australian patent AU 687863 are directed to

  • a method for transforming a scutellum of an immature embryo of a monocotyledon with Agrobacterium having a desired gene. The embryo is not submitted to a dedifferentiation process prior to transformation with Agrobacterium.

Japan Tobacco

EP 672752 B1

  • Earliest priority – 3 September 1993
  • Filed – 1 Sept 1994
  • Granted – 26 May 2004
  • Expected expiry – 31 Aug 2014
Title – Method of transforming monocotyledon by using scutellum of immature embryo

Claim 1A method for transforming monocotyledons comprising transforming scutellum of an immature embryo of a monocotyledon with a bacterium belonging to genus Agrobacterium containing a desired gene, which immature embryo has not been subjected to a dedifferentiation treatment, to obtain a transformant.

The independent claim is the same as the Australian patent.

Designated contracting States at the time of grant are: Austria, Belgium, Switzerland, Germany, Denmark, Spain, France, United Kingdom, Greece (reported on INPADOC as lapsed), Ireland, Italy, Liechtenstein, Luxembourg, Monaco (reported on INPADOC as lapsed), Netherlands, Portugal, Sweden.

EP 672752 A1

  • Earliest priority – 3 September 1993
  • Filed – 1 September 1994
  • OPI – 20 September 1995
  • Granted as EP 672752 B1 (see above)
Title – Method of transforming menocotyledon by using scutellum of immature embryo

This application has been granted as EP 672752 B1 (see above).

The independent claim in this application has been granted without amendments.

Remarks
  1. The United States application No. 428238, corresponding to a PCT application, was filed on 3 May 1995. Specification and claims of the PCT application WO 956722 are in Japanese.
  2. National phase entry of the PCT application WO 956722 in Japan (JP 3329819) has been granted on Sept 30 2002.
  3. National phase entry of the PCT application WO 956722 in Canada (CA 2148499) is still pending.

Note: Patent information on this page was last updated on 2 February 2006.