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Pepper

Summary

DNA Plant Technology has been granted a United States patent directed to the transformation of a pepper explant (young cotyledon) with A. tumefaciens or A. rhizogenes. Shoot formation is then induced from the transformed cells. Gibberellin and an inhibitor of ethylene are used as components of an elongation/selection culture medium for shoots.

Specific Patent Information

Patent Number Title, Independent Claims and Summary of Claims Assignee
US 5262316

  • Earliest priority – 22 November 1991
  • Filed – 22 November 1991
  • Granted – 16 November 1993
  • Expected expiry – 22 November 2011
 Title – Genetically transformed pepper plants and methods for their production

Claim 1A method for genetically transforming a pepper plant, said method comprising:

A) co-cultivating explant material from the pepper plant with Agrobacterium tumefaciens or rhizogenes cells carrying an exogenous DNA sequence wherein the explant material is selected from the group consisting of young embryonic cotyledons and young expanded cotyledons;
B) selecting and inducing shoots from the explant material from step (A), wherein said shoots are obtained from non-callus material and express the exogenous DNA sequence; and
C) elongating and further selecting the shoots from step (B)
Claim 11
A method for genetically transforming a pepper plant, said method comprising:A) obtaining the explant material from the pepper plant seed;
B) co-cultivating the explant material obtained in step (A) with Agrobacterium tumefaciens or rhizogenes cells carrying an exogenous DNA sequence in a co-cultivation medium for a time and under conditions selected to effect transfer of the exogenous DNA sequence to cells of the explant material obtained in step (A), wherein the explant material is selected from the group consisting of young embryonic cotyledons and young expanded cotyledons;
C) removing the Agrobacterium cells from the explant material obtained in step (A);
D) selecting and inducing shoots from the explant material from step (C) in a selection/induction medium for a time and under conditions chosen to produce shoots from non-callus regions of the explant material and select shoots which express the exogenous DNA sequence, wherein the elongation/selection medium contains a gibberellin in the amount of about 0.1 to 50 mg/l and an inhibitor of ethylene action;
E) elongating and further selecting the shoots from step (D) in an elongation/selection medium; and
F) rooting the selecting regenerated shoots from step (E) in a rooting medium.
Claim 25
A method for generating a pepper plant, said method comprising:A) obtaining young explant material from the pepper plant, wherein the young explant material is selected from the group consisting of embryonic cotyledons and young expanded cotyledons;
B) inducing shoots from the explant material from step (A) in an induction medium containing BA for a time and under conditions chosen to induce about regeneration from non-callus regions of the explant material; and
C) elongating the shoots from step (B) in an elongation medium containing a gibberellin and an inhibitor of ethylene action.
Claim 31
A method for regenerating a pepper plant, said method comprising:A) obtaining young explant material from a pepper plant seed;
B) inducing shoots from the young explant material from step (A) in an induction medium containing BA for a time and under conditions chosen to induce shoot regeneration from non-callus regions of the explant material, wherein the young explant material is selected from the group consisting of embryonic cotyledons and young expanded cotyledons;
C) elongating the shoots from step (B) in an elongation medium containing a gibberellin and an inhibitor of ethylene action; and rooting the shoots from step (C) in a rooting medium.

DNA Plant Technology
WO 1993/09665 A1

  • Earliest priority – 22  November 1991
  • Filed – 10 November 1992
  • OPI – 27 May 1993
 Title – Genetically transformed pepper plants and methods for their production

Claim 1
A method for genetically transforming a pepper plant, said method comprising:(a)  cocultivating explant material from the pepper plant with Agrobacterium cells carrying an exogenous DNA sequence;
(b) selecting and inducing shoots from the explant material from step (a), wherein said shoots are obtained from non-callus material and express the exogenous DNA sequence; and
(c) elongating and further selecting the shoots from step (b).
Claim 12
A method for genetically transforming a pepper plant, said method comprising:(a) obtaining the explant material from the pepper plant seed;
(b) cocultivating the explant material obtained in step (a) with Agrobacterium cells carrying an exogenous DNA sequence in a cocultivation medium for a time and under conditions selected to effect transfer of the exogenous DNA sequence to cells of the explant material obtained in step (a);
(c) removing the Agrobacterium cells from the explant material obtained in step (a);
(d) selecting and inducing shoots from the explant material from step (c) in a selection/induction medium for a time and under conditions chosen to produce shoots from non-callus regions of the explant material and select shoots which express the exogenous DNA sequence;
(e) elongating and further selecting the shoots from step (d) in an elongation/selection medium; and
(f) rooting the selected regenerated shoots from step
(e) in a rooting medium.
Claim 31
A pepper plant which expresses a heterologous gene.
Claim 32
A method for regenerating a pepper plant, said method comprising:(a) obtaining young explant material from the pepper plant;
(b) inducing shoots from the explant material from step (a), wherein said shoots are obtained from non-callus material; and
(c) elongating the shoots from step (b).
Claim 41
A method for regenerating a pepper plant, said method comprising:(a) obtaining young explant material from a pepper plant seed;
(b)  inducing shoots from the young explant material from step (a) in an induction medium for a time and under conditions chosen to produce shoots from non-callus regions of the explant material;
(c) elongating the shoots from step (b) in an elongation medium containing gibberelin; and
(d) rooting the shoots from step (C) in a rooting medium.
Remarks National phase entry of WO 1993/09665 in Australia (AU 30743/92) has lapsed on 11 August 1994.

Note: Patent information on this page was last updated on 21 March 2006.