The INIA1 and the IVIA2 from Spain have a granted United States patent directed to a method for transforming adult citrus plants with A. tumefacienshaving a gene of interest.
The invention discloses in vitro micrografting of transformed shoots onto stocks, at least twice, in order to generate complete adult plants. The second in vitro micrografting can be skipped by planting the first micrografted plants directly into soil.
The related European application EP 870 838 A3, which is not presented here, does not refer to a transformation method in particular, and instead inoculation of a vector carrying a gene of interest into a citrus plant can be performed by any method, including Agrobacterium as disclosed in dependent Claim 2. Furthermore, independent Claim 1 covers any woody species, therefore this application is mentioned in the section ‘Woody Tree Species’.
1 Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria (INIA), Spain
2 Instituto Valenciano de Investigaciones Agrarias (IVIA), Spain
Specific Patent Information
|Patent Number||Title, Independent Claims and Summary of Claims||Assignee|
||Title – Procedure for the genetic transformation of adult plants of woody species
Method for transforming explants of adult citrus plants with a non-oncogenic A. tumefaciens having a gene of interest. The transformed shoots are micrografted onto other stocks to allow regeneration of complete adult plants.
|INIA & IVIA|
Note: Patent information on this page was last updated on 14 March 2006.