Patent application filed by Japan Tobacco Inc.
The European application filed by Japan Tobacco discloses methods for co-transforming plants with two T-DNAs. One of the T-DNAs has a gene conferring drug-resistance and the other has a gene of interest and is part of a hybrid plasmid. The hybrid plasmid is constructed by homologous recombination of an acceptor and an intermediate plasmid.
Specific Patent Information
|Patent Number||Title, Independent Claims and Summary of Claims||Assignee|
|EP 687730 A1
||Title – Method of transforming plant and vector therefor
The claims of the application EP 687 730 A1 recite the transformation of a plant with two different T-DNAs via Agrobacterium. The first T-DNA contains a gene conferring drug resistance to the plant and the second one contains a DNA fragment of interest. In the method claim (claim 1), the second T-DNA is part of a hybrid vector formed by homologous recombination between an acceptor vector and an intermediate vector.
A hybrid vector (claim 16) harboring the first T-DNA as described and a second T-DNA with a cloning site is also formed by homologous recombination of the vectors mentioned above.
Elements of the acceptor vector and the intermediate vector are also recited in the claims.
Japan Tobacco Inc.
Note: Patent information on this page was last updated on 2 March 2006.