Weight reduction of the transformed plant tissue
Patent applications filed by Monsanto
During co-cultivation of plant tissue with Agrobacterium it is desirable to reduce the weight of the explant in order to facilitate the DNA transfer process and the formation of embryogenic callus.
Monsanto‘s disclosure teaches methods for reducing the weight of the explant during the co-cultivation period. Preferred methods of the inventors include reduction of moisture conditions, applying vacuum, increasing the osmotic potential of the media by use of mannitol, sorbitol or polyethylene glycol, air drying the explant by evaporation or applied air, or applying chemicals (desiccants) such as calcium oxide to extract moisture from the explant.
Specific Patent Information
Patent Number | Title, Independent Claims and Summary of Claims | Assignee | |
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US 2001/054186 AA
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Title – An improved efficiency Agrobacterium-mediated plant transformation method
The claims as filed of both the United States and PCT applications recite a method for producing a fertile transgenic plant by co-culturing a plant cell or tissue with Agrobacterium having the genes of interest under conditions that decrease the weight of the explant. The transformed cell lines are selected and regenerated into a fertile transgenic plant. According to the specification, the method to reduce the weight of the inoculated explant ‘comprises any method that reduces the weight of the inoculated explant and facilitates the DNA transfer process.’ Preferred methods to reduce the weight of the explant are (but not limited to):
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Monsanto | |
AU 773764
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Title – An improved efficiency Agrobacterium-mediated plant transformation method
Granted AU 773764 recites a method for producing a fertile transgenic plant using Agrobacterium, where the method of weight reduction of the inoculated explant is limited to ‘limitation or removal of water from the explant‘. |
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Note: Patent information on this page was last updated on 7 March 2006.