Woody tree species
The forestry industry –which relies on woody tree species– is a major economical factor for several countries. Tree species like Eucalyptusand Pinus are very important in the paper and cellulose industry. Because of their long generation times, novel breeding methodologies are sought to introduce disease resistance genes, influence lignin production or modulate flowering time for example. Patents covering transformation technologies could be of commercially strategic relevance.
- The European application assigned to the Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria (INIA) and theInstituto Valenciano de Investigaciones Agrarias (IVIA), both in Spain, is related to a United States patent analyzed in the ‘Citrus’ section. The present application discloses a procedure for the transformation of any woody species using adult plant material as primary explant. The explant is grafted on a stock to generate shoots which are then used as target tissue for Agrobacterium-mediated transformation. Treated shoots are then re-grafted in vitro to select for transformed shoots, which are then regenerated into full transgenic plants. This method addresses the problem of how to obtain improved clones from elite woody trees from which it is usually hard to obtain totipotent, regenerable material.
- The PCT application assigned to the Companhia Suzano de Papel e Celulose and the University of Sao Paulo in Brazil discloses a method to transform woody tree species starting from pre-germinated seed material. The method disclosed was developed for transformation of Eucalyptus spp but the claims have been worded to encompass any woody tree species, which could include conifers. The method disclosed in the independent claims is rather detailed in terms of timing of the multiple steps. This timing could be very different for other tree species than Eucalyptus, which would allow ways to design around the claim in this patent (if granted) and thus avoid infringement.
Specific Patent Information
|Patent Number||Tite, Independent Claims and Summary of Claims||Assignee|
|WO 2002/14463 A2
||Title – Method for genetic transformation of woody trees
A method for transformation of woody trees using pre-germinated seed as target tissue for Agrobacterium-mediated transformation.
|Companhia Suzano de Papel e Celulose and Universidade De Sao Paulo|
|EP 0870838 A2
||Title – Procedure for the genetic transformation of adult plants of woody species
A procedure for transformation of adult woody species with appropriate vectors, which is not limited to Agrobacterium. Adult tissue is grafted on stocks to generate new buds or apices that are used as target tissue for transformation followed by in vitro micrografting of transgenic shoots.
Note: Patent information on this page was last updated on 28 March 2006.