Immature embryo transformation
Immature embryo transformation – patents and application assigned to Japan Tobacco
Patents and application assigned to Japan Tobacco
This family of patents discloses use of an immature embryo of a monocot for Agrobacterium transformation. Within the embryo, the scutellum (name given to the single massive cotyledon (seed leaf) of monocot plants) is transformed. The scutellum is capable of producing dedifferentiated calli having the ability to regenerate normal plants after transformation.
The bacterium used for transformation contains either a Ti or Ri (root-inducing) plasmid with the desired gene and a plasmid having a virulence region derived from the A. tumefaciensTi plasmid pTiBo542.
Specific patent information
Patent Number | Title, Independent Claims and Summary of Claims | Assignee | |
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EP 672752 B1
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Title – Method of transforming monocotyledon by using scutellum of immature embryo
EP B 672 752 contains the same independent claim as the Australian patent. Designated States at the time of grant are: Austria, Belgium, Switzerland, Germany, Denmark, Spain, France, United Kingdom, Greece (reported on INPADOC as lapsed), Ireland, Italy, Liechtenstein, Luxembourg, Monaco (reported on INPADOC as lapsed), Netherlands, Portugal, Sweden |
Japan Tobacco |
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AU 687863
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Title – Method of transforming monocotyledon by using scutellum of immature embryo
The claims of the Australian patent AU-B-687 863 are directed to: a method for transformation of a scutellum of an immature embryo of a monocotyledon with Agrobacterium having a desired gene. The embryo is not subjected to a dedifferentiation process prior the transformation with Agrobacterium. |
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Note: Patent information on this page was last updated on 21 February 2006.