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Immature embryo transformation

Immature embryo transformation – patents and application assigned to Japan Tobacco

Patents and application assigned to Japan Tobacco

This family of patents discloses use of an immature embryo of a monocot for Agrobacterium transformation. Within the embryo, the scutellum (name given to the single massive cotyledon (seed leaf) of monocot plants) is transformed. The scutellum is capable of producing dedifferentiated calli having the ability to regenerate normal plants after transformation.

The bacterium used for transformation contains either a Ti or Ri (root-inducing) plasmid with the desired gene and a plasmid having a virulence region derived from the A. tumefaciensTi plasmid pTiBo542.

Specific patent information

Patent Number Title, Independent Claims and Summary of Claims Assignee
EP 672752 B1

  • Earliest priority – 3 September 1993
  • Filed – 1 September 1994
  • Granted – 26 May 2004
  • Expected expiry – 3 September 2013
 Title – Method of transforming monocotyledon by using scutellum of immature embryo

Claim 1
A method for transforming monocotyledons comprising transforming scutellum of an immature embryo of a monocotyledon with a bacterium belonging to genus Agrobacterium containing a desired gene, which immature embryo has not been subjected to a dedifferentiation treatment, to obtain a transformant.

EP B 672 752 contains the same independent claim as the Australian patent.

Designated States at the time of grant are: Austria, Belgium, Switzerland, Germany, Denmark, Spain, France, United Kingdom, Greece (reported on INPADOC as lapsed), Ireland, Italy, Liechtenstein, Luxembourg, Monaco (reported on INPADOC as lapsed), Netherlands, Portugal, Sweden

Japan Tobacco
AU 687863

  • Earliest priority – 3 September 1993
  • Filed – 1 September 1994
  • Granted – 5 March 1998
  • Expected expiry – 3 September 2013
 Title – Method of transforming monocotyledon by using scutellum of immature embryo

Claim 1
A method for transforming monocotyledons comprising transforming scutellum of an immature embryo of a monocotyledon with a bacterium belonging to genus Agrobacterium containing a desired gene, which immature embryo has not been subjected to a dedifferentiation treatment, to obtain a transformant.

The claims of the Australian patent AU-B-687 863 are directed to:

a method for transformation of a scutellum of an immature embryo of a monocotyledon with Agrobacterium having a desired gene. The embryo is not subjected to a dedifferentiation process prior the transformation with Agrobacterium.
Remarks
  1. National phase entry of WO 95/06722 in Canada (CA 2148499) is pending.
  2. National phase entry of WO 95/06722 in Japan (JP 3329819 B2) has been published as granted on 30 September 2002.

Note: Patent information on this page was last updated on 21 February 2006.